通过 CRISPR/Cas9 系统制备新型 2 型糖尿病小型猪模型。
Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system.
机构信息
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin Province, People's Republic of China.
出版信息
Cell Death Dis. 2019 Oct 28;10(11):823. doi: 10.1038/s41419-019-2056-5.
Diabetes has become one of the major noninfectious diseases that seriously endanger public health. The formation of islet amyloid polypeptide (IAPP) affects the normal physiological functions of the body, such as glucose metabolism and lipid metabolism. The mature human IAPP protein (hIAPP) has a strong tendency to misfold and is considered to be one of the major causes of amyloid changes in islets. Deposition of hIAPP is considered to be one of the leading causes of type 2 diabetes mellitus (T2DM). Miniature pigs are experimental animal models that are well suited for research on gene function and human diabetes. In our study, we obtained IAPP gene-humanized miniature pigs via the CRISPR/Cas9 system and somatic cell nuclear transfer (SCNT) technology. The hIAPP pigs can be used to further study the pathogenesis and related complications of T2DM and to lay a solid foundation for the prevention and treatment of T2DM.
糖尿病已成为严重危害公众健康的主要非传染性疾病之一。胰岛淀粉样多肽（IAPP）的形成会影响身体的正常生理功能，如葡萄糖代谢和脂质代谢。成熟的人 IAPP 蛋白（hIAPP）具有很强的错误折叠倾向，被认为是胰岛淀粉样变的主要原因之一。hIAPP 的沉积被认为是导致 2 型糖尿病（T2DM）的主要原因之一。小型猪是实验动物模型，非常适合研究基因功能和人类糖尿病。在我们的研究中，我们通过 CRISPR/Cas9 系统和体细胞核移植（SCNT）技术获得了 IAPP 基因人源化小型猪。hIAPP 猪可用于进一步研究 T2DM 的发病机制及相关并发症，并为 T2DM 的防治奠定坚实基础。
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