小鼠肾脏中多聚腺苷酸降解活性的亚细胞分布。
The subcellular distribution of poly-A-degrading activity in mouse kidney.
作者信息
Warnick C T, Lazarus H M
出版信息
Can J Biochem. 1977 Apr;55(4):485-8. doi: 10.1139/o77-068.
Most (95%) of the poly-A-degrading activity of the mouse kidney was found in the cytoplasmic fraction and only 5% was found in the nuclear fraction; 43% of the poly-A-degrading activity of the cytoplasm was found in the mitochondria, 22% in the microsomes, and 30% in the soluble fraction. Differences in activity and specificity indicate that poly A is degraded in the nucleus by enzymes that are separate and distinct from the enzymes in the cytoplasm that degrade poly A. The nuclear poly-A-degrading activity can be separated into an endonuclease with a general specificity and exonuclease, similar to one found in Ehrlich ascites tumor cells, which shows some specificity for poly A.
小鼠肾脏中大部分（95%）的多聚腺苷酸降解活性存在于细胞质部分，仅5%存在于细胞核部分；细胞质中43%的多聚腺苷酸降解活性存在于线粒体中，22%存在于微粒体中，30%存在于可溶性部分。活性和特异性的差异表明，细胞核中的多聚腺苷酸是由与细胞质中降解多聚腺苷酸的酶不同且有区别的酶降解的。细胞核中的多聚腺苷酸降解活性可分为具有一般特异性的内切核酸酶和外切核酸酶，类似于在艾氏腹水瘤细胞中发现的一种外切核酸酶，它对多聚腺苷酸表现出一定的特异性。
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