Garnier S, Magniez-Jannin F, Rasplus J-Y, Alibert P
INRA-Centre de Biologie et de Gestion des Populations, Campus International de Baillarguet, Montferrier-sur-Lez, France.
J Evol Biol. 2005 Mar;18(2):269-80. doi: 10.1111/j.1420-9101.2004.00854.x.
Population differentiation is a crucial step in the speciation process and is therefore a central subject in studies of microevolution. Assessing divergence and inferring its dynamics in space and time generally require a wide array of markers. Until now however, most studies of population structure are based on molecular markers and those concerning morphological traits are more scarce. In the present work, we studied morphological differentiation among populations of the ground beetle Carabus solieri, and tested its congruence with genetic population structure. The shape of pronotum and aedeagus was assessed using Dual Axis Fourier Shape Analysis. manova on Fourier coefficients revealed highly significant morphological variation between populations and a similar geographical pattern of differentiation for both structures. On the whole, morphological and genetic patterns were also found to be congruent. Our analysis confirms the phylogeographical scenario proposing that two entities of C. solieri differentiated during the last glaciation events before recolonizing the actual range of the species. It also indicates a large introgression between the two differentiated entities in the centre of the range.
种群分化是物种形成过程中的关键步骤，因此是微进化研究的核心主题。评估分歧并推断其在空间和时间上的动态通常需要大量的标记。然而到目前为止，大多数种群结构研究是基于分子标记，而涉及形态特征的研究则更为稀少。在本研究中，我们研究了步甲Carabus solieri种群间的形态分化，并测试了其与遗传种群结构的一致性。使用双轴傅里叶形状分析评估前胸背板和阳茎的形状。对傅里叶系数进行的多变量方差分析揭示了种群间高度显著的形态变异，以及两种结构相似的地理分化模式。总体而言，形态和遗传模式也被发现是一致的。我们的分析证实了系统地理学假说，即C. solieri的两个实体在末次冰期事件期间分化，之后重新定殖到该物种的实际分布范围。它还表明在分布范围中心的两个分化实体之间存在大量的基因渗入。
