Colak Meltem, Evcil Sinan, Bayindir Y Ziya, Yigit Nimet
Department of Endodontics of the Faculty of Dentistry at Atatürk University in Erzurum, Turkey.
J Contemp Dent Pract. 2005 Feb 15;6(1):94-106.
The in vitro reduction of a bacterial population in a root canal by mechanical instrumentation using three techniques was evaluated. Root canals inoculated with a Enterococcus faecalis (E. faecalis) suspension were instrumented using hand Hedstroem files, Giromatic files, and Hero 642 rotary instruments. Irrigation was performed using sterile saline solution. Root canals were sampled before and after instrumentation. After serial dilutions, samples were plated onto Mitis-Salivarius agar and the colony forming units grown were counted. All instruments tested were able to significantly reduce the number of bacterial cells in the root canal, however, the results of this study indicated that Hedstroem files, Giromatic, and Hero 642 techniques were not significantly different in their ability to reduce intracanal bacteria.
评估了使用三种技术通过机械预备对根管内细菌数量进行的体外减少情况。用粪肠球菌（E. faecalis）悬液接种的根管，分别使用手动Hedstroem锉、Giromatic锉和Hero 642旋转器械进行预备。使用无菌盐溶液进行冲洗。在预备前后对根管进行取样。经过系列稀释后，将样本接种到米氏-唾液琼脂上，并对生长的菌落形成单位进行计数。所有测试的器械都能够显著减少根管内的细菌细胞数量，然而，本研究结果表明，Hedstroem锉、Giromatic锉和Hero 642技术在减少根管内细菌的能力方面没有显著差异。
