吲哚美辛诱导的胃溃疡的核磁共振分析

Nuclear magnetic resonance analysis of indomethacin-induced gastric ulcers.

作者信息

Ramadan Saadallah, Bonin Antonio M, Kennedy Brendan J, Hambley Trevor W, Lay Peter A

机构信息

Centre for Heavy Metals Research, School of Chemistry, University of Sydney, NSW, 2006, Australia.

出版信息

Chem Res Toxicol. 2005 Feb;18(2):123-8. doi: 10.1021/tx049806t.

DOI:10.1021/tx049806t

PMID:15720115

Abstract

Acetonitrile extracts of ulcerated and control rat stomachs were studied by various NMR techniques in an attempt to understand how indomethacin, a common and powerful nonsteroidal antiinflammatory drug (NSAID), induces ulcers in the stomach. One- (1D) and two-dimensional (2D) NMR spectra of extracts of ulcerated and control stomachs revealed that glycolytic and Krebs cycle enzymes were partially inhibited in the ulcerated stomach as shown by the lactate/glucose ratio. The (total choline)/lactate ratio was also higher in the extract from the control stomach than in the ulcerated stomach. Glycerophosphoethanolamine and glycerophosphocholine concentrations were higher in the ulcerated stomach extract as compared with the control stomach extract. These results explain the gastrointestinal protective effect of D-glucose and Krebs cycle intermediates on NSAID-induced ulceration.

摘要

通过各种核磁共振技术研究了溃疡大鼠和对照大鼠胃的乙腈提取物，以试图了解一种常见且强效的非甾体抗炎药（NSAID）吲哚美辛如何诱发胃溃疡。溃疡胃和对照胃提取物的一维（1D）和二维（2D）核磁共振谱显示，如乳酸/葡萄糖比值所示，溃疡胃中的糖酵解酶和三羧酸循环酶受到部分抑制。对照胃提取物中的（总胆碱）/乳酸比值也高于溃疡胃。与对照胃提取物相比，溃疡胃提取物中甘油磷酸乙醇胺和甘油磷酸胆碱的浓度更高。这些结果解释了D-葡萄糖和三羧酸循环中间体对NSAID诱导溃疡的胃肠道保护作用。