Chami N, Bennis S, Chami F, Aboussekhra A, Remmal A
Faculté des Sciences de Fès, Laboratoire de Biotechnologie, Atlas Fes, Morocco.
Oral Microbiol Immunol. 2005 Apr;20(2):106-11. doi: 10.1111/j.1399-302X.2004.00202.x.
BACKGROUND/AIMS: The mechanism of the anticandidal action of the major phenolic components of oregano and clove essential oils - carvacrol and eugenol - was studied. This activity was also evaluated for the therapeutic efficacy in the treatment of the experimental oral candidiasis induced by Candida albicans in immunosuppressed rats.
In vitro, the addition of carvacrol at 0.1% or eugenol at 0.2% during the exponential growth of C. albicans was evaluated. The release of substances absorbing at 280 nm by cells treated with these two components was also measured spectrophotometrically. In vivo, oral candidiasis in immunosuppressed rats was established by inoculating 3 x 10(8) cells of C. albicans with a cotton swab on three alternate days. The number of colony counts was evaluated from the oral cavity of rats treated for eight consecutive days with carvacrol, eugenol or nystatin and compared to untreated controls.
Carvacrol and eugenol were fungicidal in exponentially growing C. albicans. Interestingly, this fungicidal effect was accompanied by the release of substances absorbing at 280 nm. In an immunosuppressed rat model of oral candidiasis, carvacrol or eugenol treatment significantly (P < 0.05) reduced the number of colony counts sampled from the oral cavity of rats treated for eight consecutive days compared to untreated control rats. Similar results were obtained with nystatin used as a reference treatment.
The in vitro results indicated that both carvacrol and eugenol exerted an anticandidal effect by a mechanism implicating an important envelope damage. Their in vivo efficacy on experimental oral candidiasis leads us to consider them as possible antifungal agents.
背景/目的：研究牛至和丁香精油的主要酚类成分香芹酚和丁香酚的抗念珠菌作用机制。还评估了这种活性在治疗免疫抑制大鼠由白色念珠菌引起的实验性口腔念珠菌病中的治疗效果。
在体外，评估在白色念珠菌指数生长期添加0.1%香芹酚或0.2%丁香酚的情况。还用分光光度法测量了用这两种成分处理的细胞释放的在280nm处有吸收的物质。在体内，通过用棉签每隔一天接种3×10⁸个白色念珠菌细胞来建立免疫抑制大鼠的口腔念珠菌病。对连续八天用香芹酚、丁香酚或制霉菌素治疗的大鼠口腔进行菌落计数评估，并与未治疗的对照组进行比较。
香芹酚和丁香酚对处于指数生长期的白色念珠菌具有杀菌作用。有趣的是，这种杀菌作用伴随着在280nm处有吸收的物质的释放。在口腔念珠菌病的免疫抑制大鼠模型中，与未治疗的对照大鼠相比，香芹酚或丁香酚治疗显著（P<0.05）减少了连续八天治疗的大鼠口腔取样的菌落计数。用作参考治疗的制霉菌素也获得了类似结果。
体外结果表明，香芹酚和丁香酚均通过涉及重要包膜损伤的机制发挥抗念珠菌作用。它们对实验性口腔念珠菌病的体内疗效使我们将它们视为可能的抗真菌剂。
