Stafstrom C E, Thompson J L, Holmes G L
Department of Neurology, Children's Hospital, Boston, MA 02115.
Brain Res Dev Brain Res. 1992 Feb 21;65(2):227-36. doi: 10.1016/0165-3806(92)90184-x.
Acute and chronic effects of seizures induced by intraperitoneal (i.p.) injection of kainic acid (KA) were studied in developing rats (postnatal days (P) 5, 10, 20, 30, and adult 60). For 3 months following KA-induced status epilepticus, spontaneous recurrent seizure (SRS) occurrence was quantified using intermittent video monitoring. Latency to generalized seizures was then tested using flurothyl, and brains were histologically analyzed for CA3 lesions. In P5-10 rats, KA caused generalized tonic-clonic ('swimming') seizures. SRS did not develop, and there was no significant difference between control and KA-treated rats in latency to flurothyl-induced seizures. In contrast, rats P20 and older exhibited limbic automatisms followed by limbic motor seizures which secondarily generalized. Incidence and frequency of SRS increased with age. P20-30 rats with SRS had shorter latencies to flurothyl seizures than did KA-treated P20-30 rats without SRS or controls. KA-treated P60 rats (with or without SRS) had shorter latencies than controls to flurothyl seizure onset. SRS in P60 rats occurred sooner after KA than in P20-30 rats. CA3 lesions were seen in P20-60 rats with and without SRS, but not in P5-10 rats. These data suggest that there are developmental differences in both acute and chronic responses to KA, with immature animals relatively protected from the long-term deleterious effects of this convulsant.
研究了腹腔注射海藻酸（KA）诱导的癫痫发作对发育中大鼠（出生后第5、10、20、30天以及成年60天）的急性和慢性影响。在KA诱导的癫痫持续状态后的3个月内，使用间歇性视频监测对自发性反复癫痫发作（SRS）的发生情况进行量化。然后使用三氟乙烷测试全身性癫痫发作的潜伏期，并对大脑进行组织学分析以检测CA3区病变。在出生后第5 - 10天的大鼠中，KA引发全身性强直阵挛性（“游泳”）癫痫发作。未出现SRS，在三氟乙烷诱导的癫痫发作潜伏期方面，对照组和KA处理组大鼠之间无显著差异。相比之下，出生后第20天及以上的大鼠表现出边缘性自动症，随后是边缘性运动性癫痫发作，继而全身性发作。SRS的发生率和频率随年龄增加。有SRS的出生后第20 - 30天大鼠比无SRS的KA处理组出生后第20 - 30天大鼠或对照组对三氟乙烷癫痫发作的潜伏期更短。KA处理的成年60天大鼠（有或无SRS）比对照组对三氟乙烷癫痫发作起始的潜伏期更短。成年60天大鼠中的SRS在KA注射后比出生后第20 - 30天大鼠出现得更早。在有和无SRS的出生后第20 - 60天大鼠中均可见CA3区病变，但在出生后第5 - 10天大鼠中未见。这些数据表明，对KA的急性和慢性反应存在发育差异，未成熟动物相对免受这种惊厥剂的长期有害影响。
