盘尾丝虫病患者的IgG及IgG亚类情况
The profile of IgG and IgG subclasses of onchocerciasis patients.
作者信息
Dafa'alla T H, Ghalib H W, Abdelmageed A, Williams J F
机构信息
US NIH/Sudan Medical Parasitology Research Project, Khartoum, Sudan.
出版信息
Clin Exp Immunol. 1992 May;88(2):258-63. doi: 10.1111/j.1365-2249.1992.tb03070.x.
In this study Onchocerca gutturosa was compared with O. volvulus in an ELISA test to detect Onchocerca-specific IgG and IgG subclasses. The test was developed and standardized to detect Onchocerca-specific IgG and IgG subclasses in sera of onchocerciasis patients and endemic controls. Onchocerca volvulus and O. gutturosa crude water-soluble antigens showed no significant difference in detecting onchocerca-specific IgG antibody (T = 1.88, P greater than 0.05). The levels of IgG subclasses varied greatly. IgG4 showed the highest detected mean level (0.84 +/- 0.59) and the other three subclasses showed considerably lower mean levels (IgG1 = 0.27 +/- 0.16, IgG2 = 0.24 +/- 0.17, IgG3 = 0.28 +/- 0.12). The status and score of skin lesions were found to have significant effect on the IgG and IgG subclasses levels (all P less than 0.001). IgG4 showed a positive correlation with the microfilarial (Mf) load (r = 0.21, P less than 0.03). IgG3 levels have a significant negative correlation with the Mf load (r = -0.23, P less than 0.02). The biological significance of these IgG and IgG subclasses in onchocerciasis is discussed.
在本研究中，在酶联免疫吸附测定（ELISA）试验中对牛盘尾丝虫与旋盘尾丝虫进行比较，以检测盘尾丝虫特异性IgG及IgG亚类。该试验经过开发和标准化，用于检测盘尾丝虫病患者和流行区对照血清中的盘尾丝虫特异性IgG及IgG亚类。旋盘尾丝虫和牛盘尾丝虫的粗水溶性抗原在检测盘尾丝虫特异性IgG抗体方面无显著差异（T = 1.88，P＞0.05）。IgG亚类水平差异很大。IgG4检测到的平均水平最高（0.84±0.59），其他三个亚类的平均水平则低得多（IgG1 = 0.27±0.16，IgG2 = 0.24±0.17，IgG3 = 0.28±0.12）。发现皮肤病变的状态和评分对IgG及IgG亚类水平有显著影响（所有P＜0.001）。IgG4与微丝蚴（Mf）负荷呈正相关（r = 0.21，P＜0.03）。IgG3水平与Mf负荷呈显著负相关（r = -0.23，P＜0.02）。本文讨论了这些IgG及IgG亚类在盘尾丝虫病中的生物学意义。
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