从用作伤口愈合剂的人胎盘提取物中分离Ⅲ型纤连蛋白样肽。
Isolation of fibronectin type III like peptide from human placental extract used as wound healer.
作者信息
Chakraborty Piyali Datta, Bhattacharyya Debasish
机构信息
Department of Drug Design, Development and Molecular Modeling, Indian Institute of Chemical Biology, 4 Raja S.C. Mallick Road, Jadavpur, Calcutta, West Bengal 700032, India.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Apr 15;818(1):67-73. doi: 10.1016/j.jchromb.2004.09.059.
A peptide of around 7.4 kDa has been purified from the aqueous extract of human placenta used as wound healer. Derived partial amino acid sequence from mass spectrometric analysis showed its homology with human fibronectin type III. Under nondenaturing condition, it formed aggregate, the elution pattern of which from reverse-phase HPLC was identical with that of fibronectin type III. Immuno-blot of the peptide with reference fibronectin type III-C showed strong cross reactivity. Since fibronectin type III plays important roles in wound healing, similar peptide in the extract is likely to take part in curing process.
一种约7.4 kDa的肽已从用作伤口愈合剂的人胎盘水提取物中纯化出来。质谱分析得出的部分氨基酸序列显示其与人类III型纤连蛋白具有同源性。在非变性条件下，它形成聚集体，其从反相高效液相色谱的洗脱模式与III型纤连蛋白相同。该肽与参考III型纤连蛋白-C的免疫印迹显示出强交叉反应性。由于III型纤连蛋白在伤口愈合中起重要作用，提取物中的类似肽可能参与治愈过程。
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