Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, Haidian, Beijing 100083, China.
Beijing Laboratory of Food Quality and Safety, China Agricultural University, No. 17 Qinghua East Road, Haidian, Beijing 100083, China.
Molecules. 2018 Dec 21;24(1):28. doi: 10.3390/molecules24010028.
Though the biological effects of human placental extract have been widely studied, it has limited availability and its use poses ethical problems. Thus, domestic animal placental extracts are suggested as alternatives. In this study, the protective effect of sheep placental extract (SPE) on concanavalin A (Con A)-induced liver injury was investigated. BALB/c mice were randomly divided into six groups, including one normal group and five experimental groups, which received different oral doses of SPE (0, 5, 10 and 50 mg/kg) or a mixture of amino acids for 3 days before Con A injection. Compared with Con A-induced model group, the SPE administration significantly decreased serum aminotransaminase activity, alleviated pathological changes, recovered liver antioxidant capacity and prevented the increase of nitric oxide. Secretion of pro-inflammatory cytokines in serum decreased and mRNA expression of hepatic intercellular adhesion molecule-1, interferon-inducible chemokine 10 and inducible nitric oxide synthase were downregulated, while B-cell lymphoma-2 expression increased. The administration of amino acids mixture had no significant effect in most measurements compared with the model group, which indicated proteins and peptides, rather than individual amino acid, were largely responsible for the bioactivity of SPE. The results indicate SPE has potential therapeutic effects against immune-mediated hepatitis.
虽然人类胎盘提取物的生物学效应已被广泛研究，但由于其可用性有限且存在伦理问题，因此建议使用家畜胎盘提取物作为替代品。本研究探讨了绵羊胎盘提取物（SPE）对刀豆蛋白 A（Con A）诱导的肝损伤的保护作用。BALB/c 小鼠随机分为六组，包括一组正常组和五组实验组，实验组在 Con A 注射前连续 3 天分别给予不同剂量的 SPE（0、5、10 和 50mg/kg）或氨基酸混合物。与 Con A 诱导的模型组相比，SPE 给药可显著降低血清转氨酶活性，减轻病理变化，恢复肝抗氧化能力，防止一氧化氮增加。血清中促炎细胞因子的分泌减少，肝细胞间黏附分子-1、干扰素诱导的趋化因子 10 和诱导型一氧化氮合酶的 mRNA 表达下调，而 B 细胞淋巴瘤-2 的表达增加。与模型组相比，氨基酸混合物的给药在大多数测量中没有显著效果，这表明 SPE 的生物活性主要归因于蛋白质和肽，而不是单个氨基酸。研究结果表明 SPE 对免疫介导的肝炎具有潜在的治疗作用。
