McNamara C J, Perry T D, Bearce K, Hernandez-Duque G, Mitchell R
Laboratory of Microbial Ecology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
J Microbiol Methods. 2005 May;61(2):245-50. doi: 10.1016/j.mimet.2004.12.004. Epub 2004 Dec 30.
Limestone and marble have been used extensively in the construction of modern and historic buildings. Microbial colonization and growth on these stone structures is common. Microbial deterioration of stone has been assessed by measuring Ca2+ released from the stone, using ion selective electrodes and titration with EDTA. In this study, the calcium binding fluorochrome Rhod-5N was used to measure Ca2+ released from limestone by endolithic bacteria as an indicator of biodeterioration. In a 17 d flask experiment, Ca2+ released by endolithic bacteria was twice that of uninoculated controls. Rhod-5N is a rapid and accurate method for measuring microbial biodeterioration of stone.
石灰石和大理石已广泛应用于现代和历史建筑的建造中。这些石材结构上的微生物定殖和生长很常见。通过使用离子选择性电极测量石材释放的Ca2+以及用EDTA滴定来评估石材的微生物劣化。在本研究中，钙结合荧光染料Rhod-5N被用作测量内生细菌从石灰石中释放的Ca2+的指标，以此作为生物劣化的指示。在一项为期17天的烧瓶实验中，内生细菌释放的Ca2+是未接种对照的两倍。Rhod-5N是一种快速、准确的测量石材微生物生物劣化的方法。
