Soibinet Mathieu, Souchon Vincent, Leray Isabelle, Valeur Bernard
Laboratoire de Chimie Générale, Conservatoire National des Arts et Métiers, 292 rue Saint-Martin, F-75141, Paris Cedex 03, France.
J Fluoresc. 2008 Nov;18(6):1077-82. doi: 10.1007/s10895-008-0352-z. Epub 2008 May 17.
The photophysical and complexing properties of Rhod-5N (commercially available) in MOPS buffer are reported. This fluorescent molecular sensor consists of a BAPTA chelating moiety bound to a rhodamine fluorophore. Its fluorescence quantum yield is low and a drastic enhancement of fluorescence intensity upon cation binding was observed. Special attention was paid to the complexation with Cd2+, a well known toxic metal ion. Possible interference with other metal ions (Na+, K+, Mg2+, Ca2+, Zn2+, Pb2+) was examined. Rhod-5N was found to be highly selective of Cd2+ over those interfering cations except Pb2+. The limit of detection is 3.1 microg l(-1).
报道了Rhod-5N（市售）在MOPS缓冲液中的光物理和络合性质。这种荧光分子传感器由与罗丹明荧光团结合的BAPTA螯合部分组成。其荧光量子产率较低，并且观察到阳离子结合后荧光强度急剧增强。特别关注了与著名的有毒金属离子Cd2+的络合。研究了与其他金属离子（Na+、K+、Mg2+、Ca2+、Zn2+、Pb2+）可能的干扰。发现Rhod-5N对Cd2+的选择性高于除Pb2+之外的那些干扰阳离子。检测限为3.1微克/升（-1）。
