Burns A R, James A L, Greene S G, Schellenberg R R, Hogg J C
University of British Columbia Pulmonary Research Laboratory, Vancouver, Canada.
Exp Lung Res. 1992 Mar-Apr;18(2):287-98. doi: 10.3109/01902149209031686.
The effect of sensory nerve C fiber network degeneration on airways permeability and responsiveness was studied in guinea pigs. Degeneration of the C fiber sensory network was produced by injecting capsaicin in solvent (total dose 50 mg/kg) into neonatal animals (n = 29) with littermate animals (n = 30) treated with solvent alone serving as controls. Sensory nerve depletion was established by comparing tracheal strips from both groups of animals in vitro. When the animals reached between 400 and 500 g body weight, airways permeability to fluorescein isothiocyanate dextran (FITC-D) and airways responsiveness to a graded dose of aerosolized histamine were measured. The data show that degeneration of the C fiber network had no effect on the airways responsiveness to histamine. They also show that degeneration of the C fiber network produced a significant (P less than or equal to .01) increase in airways reactivity [0.36 +/- 0.08 cm H2O/mL s-1 (mean +/- SEM)] following cigarette smoke exposure compared with control (0.11 +/- 0.04) or control-smoke-exposed animals (0.10 +/- 0.02). Degeneration of the C fiber network did not affect the increased permeability seen following cigarette smoke exposure (capsaicin pretreated 39.8 +/- 9.1 and control pretreated 28.4 +/- 7.9 (micrograms)(min)/mg). We conclude that degeneration of the C fiber network is associated with increased rather than decreased airways responsiveness in guinea pigs exposed to cigarette smoke.
在豚鼠中研究了感觉神经C纤维网络退化对气道通透性和反应性的影响。通过向新生动物（n = 29）注射溶于溶剂的辣椒素（总剂量50 mg/kg）来诱导C纤维感觉网络的退化，将同窝动物（n = 30）仅用溶剂处理作为对照。通过体外比较两组动物的气管条来确定感觉神经耗竭。当动物体重达到400至500 g时，测量气道对异硫氰酸荧光素葡聚糖（FITC-D）的通透性以及气道对分级剂量雾化组胺的反应性。数据显示，C纤维网络的退化对气道对组胺的反应性没有影响。数据还显示，与对照（0.11±0.04）或对照烟雾暴露动物（0.10±0.02）相比，C纤维网络的退化导致香烟烟雾暴露后气道反应性显著（P≤0.01）增加[0.36±0.08 cm H2O/mL s-1（平均值±标准误）]。C纤维网络的退化并未影响香烟烟雾暴露后观察到的通透性增加（辣椒素预处理组为39.8±9.1，对照预处理组为28.4±7.9（微克）（分钟）/毫克）。我们得出结论，在暴露于香烟烟雾的豚鼠中，C纤维网络的退化与气道反应性增加而非降低有关。
