Hulbert W M, McLean T, Hogg J C
Am Rev Respir Dis. 1985 Jul;132(1):7-11. doi: 10.1164/arrd.1985.132.1.7.
This study compared airways reactivity before and after an inflammatory response was induced in the airways by exposing them to cigarette smoke. Airways reactivity was measured to graded doses of histamine using a standard technique in 24 animals without drug pretreatment, in 17 pretreated with propranolol, in 17 pretreated with atropine, and in 18 pretreated with both atropine and propranolol. In each group, at least 5 animals not exposed to cigarette smoke served as controls, and the remaining animals were studied in groups of 4 or more at 30 min, 6, and 24 h after exposure to 100 puffs of whole cigarette smoke. The data show that airways reactivity was increased at 30 min but not at 6 and 24 h after exposure to cigarette smoke. Pretreatment with propranolol or the combination of propranolol and atropine prior to the histamine dose-response curve did not affect the increased response seen after cigarette smoke exposure, but pretreatment with atropine abolished it. Comparing this study with our previous histologic studies of the airways in similarly exposed animals, we conclude that the observed increase in airways reactivity occurs during the fluid exudative phase of the inflammatory response before the polymorphonuclear leukocytes migrate into the epithelium.
本研究比较了通过让气道暴露于香烟烟雾诱导炎症反应前后的气道反应性。采用标准技术，对24只未进行药物预处理的动物、17只预先用普萘洛尔处理的动物、17只预先用阿托品处理的动物以及18只预先用阿托品和普萘洛尔联合处理的动物，测定其对不同剂量组胺的气道反应性。在每组中，至少5只未暴露于香烟烟雾的动物作为对照，其余动物在暴露于100口整支香烟烟雾后的30分钟、6小时和24小时，以每组4只或更多只的方式进行研究。数据显示，暴露于香烟烟雾后30分钟气道反应性增加，但在6小时和24小时时未增加。在组胺剂量反应曲线之前用普萘洛尔或普萘洛尔与阿托品联合预处理，并不影响香烟烟雾暴露后出现的反应性增加，但用阿托品预处理可消除这种增加。将本研究与我们之前对类似暴露动物气道的组织学研究进行比较，我们得出结论，观察到的气道反应性增加发生在炎症反应的液体渗出期，即在多形核白细胞迁移到上皮之前。
