Branski Ryan C, Sandulache Vlad C, Dohar Joseph E, Hebda Patricia A
Otolaryngology Wound Healing Laboratory, Department of Pediatric Otolaryngology, Children's Hospital of Pittsburgh, PA 15213, USA.
Arch Otolaryngol Head Neck Surg. 2005 Feb;131(2):153-7. doi: 10.1001/archotol.131.2.153.
To build on work in laryngology and oral biology that suggests utility in the assay of secretions collected from wound sites as a predictive instrument to determine which infants will likely develop subglottic stenosis following endotracheal intubation and to document and describe the wound-healing process.
Prospective, randomized design.
Laser-induced subglottic injury was established in 3 rabbits. Secretions were collected from the subglottic region at 6 time points from days 4 to 21 following injury and from 4 uninjured control airways. The secretions were then subjected to enzyme-linked immunoassays for interleukin 1 beta and prostaglandin E2.
Three adult New Zealand white rabbits.
Interleukin 1 beta and prostaglandin E2 levels.
Measurable amounts of both mediators were obtained. Furthermore, different temporal patterns of expression were observed with interleukin 1 beta, showing increased levels on days 4 to 18, and with prostaglandin E2, showing increased levels on days 7 to 18. These results concur with emerging data regarding the role of each mediator in the wound-healing process.
Although in its infancy, the analysis of secretions collected from the site of injury in the subglottis may have utility in the management of patients following intubation-related trauma.
基于喉科学和口腔生物学方面的研究工作，该研究表明对从伤口部位收集的分泌物进行检测，作为一种预测工具，可用于确定哪些婴儿在气管插管后可能发生声门下狭窄，并记录和描述伤口愈合过程。
前瞻性随机设计。
对3只兔子造成激光诱导的声门下损伤。在损伤后第4天至21天的6个时间点，从声门下区域收集分泌物，并从4个未受伤的对照气道收集分泌物。然后对分泌物进行白细胞介素1β和前列腺素E2的酶联免疫分析。
3只成年新西兰白兔。
白细胞介素1β和前列腺素E2水平。
获得了两种介质的可测量量。此外，观察到白细胞介素1β有不同的时间表达模式，在第4天至18天水平升高，前列腺素E2在第7天至18天水平升高。这些结果与关于每种介质在伤口愈合过程中作用的新数据一致。
尽管尚处于初期阶段，但对声门下损伤部位收集的分泌物进行分析，可能有助于管理插管相关创伤后的患者。
