Hashimoto Makoto, Komatsu Haruhiko, Kozone Ikuko, Kawaide Hiroshi, Abe Hiroshi, Natsume Masahiro
Department of Plant Protection, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Biosci Biotechnol Biochem. 2005 Feb;69(2):315-20. doi: 10.1271/bbb.69.315.
The biosynthesis of pamamycin-607 (PM-607), a sixteen-membered macrodiolide compound, was studied with 13C- and 15N-labeled precursor units in Streptomyces alboniger. Feeding experiments with 13C-labeled acetate or propionate indicate that the carbon skeleton of PM-607 was derived from six acetate, four propionate and three succinate units. MS analyses of 15N-labeled PM-607 suggest that the nitrogen atom in PM-607 was derived from the alpha-amino group of an amino acid.
在白色链霉菌中，使用13C和15N标记的前体单元研究了十六元大环二酯化合物帕马霉素-607（PM-607）的生物合成。用13C标记的乙酸盐或丙酸盐进行的饲喂实验表明，PM-607的碳骨架来自六个乙酸盐、四个丙酸盐和三个琥珀酸盐单元。对15N标记的PM-607的质谱分析表明，PM-607中的氮原子来自氨基酸的α-氨基。
