长双歧杆菌中pTB6的结构与功能分析
Structural and functional analysis of pTB6 from Bifidobacterium longum.
作者信息
Tanaka Kunihito, Samura Kazuya, Kano Yasunobu
机构信息
Department of Molecular Genetics, Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Japan.
出版信息
Biosci Biotechnol Biochem. 2005 Feb;69(2):422-5. doi: 10.1271/bbb.69.422.
The complete nucleotide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB aa sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44, suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2.5 x 10(6) transformants/microg DNA.
测定了来自长双歧杆菌的pTB6（3624 bp）的完整核苷酸序列。该质粒在核苷酸（nuc）序列上与滚环复制（RCR）质粒pKJ36和pB44具有95%的同源性，在RepB氨基酸序列上也有92%的同源性，这表明pTB6通过滚环机制进行复制。检测到的推定MembB、MobA和来自orf（Orf）I的蛋白质编码对于质粒复制并非必需。我们从pTB6和pUC18构建了一个不可移动的穿梭载体，该载体以2.5×10⁶转化子/μg DNA的高效率转化长双歧杆菌。
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