Murthy Mala, Ranjan Ravi, Denef Natalie, Higashi Misao E L, Schupbach Trudi, Schwarz Thomas L
Division of Neuroscience, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
J Cell Sci. 2005 Mar 15;118(Pt 6):1139-50. doi: 10.1242/jcs.01644. Epub 2005 Feb 22.
To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.
为了详细分析多细胞生物中外泌体复合物的功能，我们在果蝇中产生了sec6突变体。我们利用这些突变来比较sec6和sec5在卵巢和神经系统中的表型，发现它们相似。我们还发现Sec5在sec6突变体中定位错误。此外，我们构建了一个带有表位标签的Sec8，它与Sec5共定位于卵母细胞膜上，并且在sec5和sec6生殖系克隆中定位错误。该构建体进一步揭示了sec8和sec5之间的遗传相互作用。综合这些数据，它们提供了关于外泌体复合物组织的新信息，并表明Sec5、Sec6和Sec8作为一个复合物发挥作用，每个成员的正确定位和功能都依赖于其他成员。
