Stewart James Bruce, Beckenbach Andrew T
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.
Genome. 2005 Feb;48(1):46-54. doi: 10.1139/g04-090.
We present the complete mitochondrial genome sequence of the meadow spittlebug Philaenus spumarius (Auchenorrhyncha: Cercopoidae). This contribution represents the second mitochondrial genome from the Hemiptera and the second of the three hemipteran suborders sampled. The genome is a circular molecule of 16 324 bp with a total A+T content of 77.0% and 76.7% for coding regions only. The gene content, order, and structure are consistent with the Drosophila yakuba genome structure (Clary and Wolstenholme 1985) and the hypothesized ancestral arthropod genome arrangement (Crease 1999). Nucleotide composition and codon usage are near the means observed in other insect mitochondria sequenced to date but have a higher A+T richness compared with the other hemipteran example, the kissing bug Triatoma dimidiata (Dotson and Beard. 2001. Insect Mol. Biol. 10: 205-215). The major noncoding region (the A+T rich region or putative control region) between the small ribosomal subunit and the tRNAIle gene includes two extensive repeat regions. The first repeat region includes 19 tandem repeats of a 46-bp sequence, whereas the second contains a longer sequence (146 bp) tandemly repeated four times.
我们公布了草地沫蝉（Philaenus spumarius，半翅目：沫蝉科）完整的线粒体基因组序列。这项研究成果代表了半翅目昆虫的第二个线粒体基因组，也是已测序的半翅目三个亚目中第二个被测序的亚目。该基因组是一个16324 bp的环状分子，仅编码区的A+T总含量就达到77.0%，整体含量为76.7%。其基因组成、排列顺序和结构与雅库布果蝇（Drosophila yakuba）的基因组结构（Clary和Wolstenholme，1985）以及推测的节肢动物祖先基因组排列方式（Crease，1999）一致。核苷酸组成和密码子使用情况与迄今已测序的其他昆虫线粒体相近，但与另一个半翅目昆虫——吸血猎蝽（Triatoma dimidiata）相比，其A+T含量更高（Dotson和Beard，2001年，《昆虫分子生物学》10：205 - 215）。小核糖体亚基和tRNAIle基因之间的主要非编码区（富含A+T的区域或假定的控制区）包含两个广泛的重复区域。第一个重复区域包含19个46 bp序列的串联重复，而第二个包含一个较长的序列（146 bp），串联重复了4次。
