Lin Wei, Jing Naihe, Basson M Albert, Dierich Andrée, Licht Jonathan, Ang Siew-Lan
Medical Research Council, National Institute for Medical Research, London, UK.
Genesis. 2005 Mar;41(3):110-5. doi: 10.1002/gene.20103.
Sef and Sprouty proteins function as feedback antagonists of fibroblast growth factor (Fgf) signaling in zebrafish embryos. To study the role of Sef in mice, we generated Sef homozygous mutant animals. These animals are viable and show normal expression of mid-hindbrain genes at embryonic days 8.5 and 9.5. To investigate the possibility of functional synergism between Sef and Sprouty proteins, we electroporated Sprouty2(Y55A), which functions in a dominant-negative manner in tissue culture cells into the mid-hindbrain region of wildtype and Sef mutant embryos. The expression pattern of Gbx2, a downstream target of Fgf signaling, was expanded or shifted in electroporated embryos, and this effect was significantly enhanced in the Sef mutant background. Altogether, our results demonstrate that Sef and Sproutys function synergistically to regulate Gbx2 expression in the anterior hindbrain.
Sef和Sprouty蛋白在斑马鱼胚胎中作为成纤维细胞生长因子（Fgf）信号的反馈拮抗剂发挥作用。为了研究Sef在小鼠中的作用，我们构建了Sef纯合突变动物。这些动物能够存活，并且在胚胎第8.5天和第9.5天时中后脑基因表达正常。为了研究Sef和Sprouty蛋白之间功能协同作用的可能性，我们将在组织培养细胞中以显性负性方式发挥作用的Sprouty2（Y55A）电穿孔导入野生型和Sef突变胚胎的中后脑区域。Fgf信号下游靶点Gbx2的表达模式在电穿孔胚胎中发生了扩展或偏移，并且在Sef突变背景下这种效应显著增强。总之，我们的结果表明Sef和Sprouty蛋白协同调节后脑前部Gbx2的表达。
