de Lumen Ben O
Department of Nutritional Sciences and Toxicology, University of California, 231 Morgan Hall, Berkeley, CA 94720-3104, USA.
Nutr Rev. 2005 Jan;63(1):16-21. doi: 10.1111/j.1753-4887.2005.tb00106.x.
Lunasin is a novel, cancer-preventive peptide whose efficacy against chemical carcinogens and oncogenes has been demonstrated in mammalian cells and in a skin cancer mouse model. Isolated and characterized in soy, lunasin peptide is also documented in barley. Lunasin is found in all of the genotypes analyzed from the US soy germ plasm collection and in commercially available soy proteins. Pilot studies show that lunasin is bioavailable in mice and rats when orally ingested, opening the way for dietary administration in cancer prevention studies. Lunasin internalizes into mammalian cells within minutes of exogenous application, and localizes in the nucleus after 18 hours. It inhibits acetylation of core histones in mammalian cells. In spite of its cancer-preventive properties, lunasin does not affect the growth rate of normal and established cancer cell lines. An epigenetic mechanism of action is proposed whereby lunasin selectively kills cells being transformed or newly transformed by binding to deacetylated core histones exposed by the transformation event, disrupting the dynamics of histone acetylation-deacetylation and leading to cell death.
芦那辛是一种新型的防癌肽，其对化学致癌物和致癌基因的功效已在哺乳动物细胞和皮肤癌小鼠模型中得到证实。芦那辛肽在大豆中被分离和鉴定，大麦中也有相关记载。在美国大豆种质资源库分析的所有基因型以及市售大豆蛋白中都能发现芦那辛。初步研究表明，芦那辛经口服摄入后在小鼠和大鼠体内具有生物利用性，为癌症预防研究中的饮食给药开辟了道路。芦那辛在外源应用后几分钟内即可内化进入哺乳动物细胞，并在18小时后定位于细胞核。它可抑制哺乳动物细胞中核心组蛋白的乙酰化。尽管芦那辛具有防癌特性，但它并不影响正常细胞系和已建立的癌细胞系的生长速度。有人提出了一种表观遗传作用机制，即芦那辛通过与转化事件暴露的去乙酰化核心组蛋白结合，选择性地杀死正在转化或新转化的细胞，破坏组蛋白乙酰化 - 去乙酰化的动态平衡，从而导致细胞死亡。
