Jeong Hyung Jin, Jeong Jin Boo, Kim Dae Seop, Park Jae Ho, Lee Jung Bok, Kweon Dae-Hyuk, Chung Gyu Young, Seo Eul Won, de Lumen Ben O
College of Natural Sciences, Andong National University, Andong, Republic of Korea.
Cancer Lett. 2007 Sep 18;255(1):42-8. doi: 10.1016/j.canlet.2007.03.022. Epub 2007 May 3.
Lunasin is a unique 43-amino acid cancer preventive peptide initially reported in soybean and barley and has been shown to be chemopreventive in mammalian cells and in a skin cancer mouse model against oncogenes and chemical carcinogens. We report here the core histone H3- and H-acetylation inhibitory properties of lunasin from wheat, a new source of the peptide and from the livers of rats fed with lunasin-enriched wheat (LEW) to measure bioavailability. A non-radioactive histone acetyl transferase assay was used to measure inhibition of core histone acetylation. The presence of lunasin in wheat was established by Western blot and identified by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). Lunasin isolated from wheat seeds at different stages of development inhibited core histone H3 and H4 acetylation in a dose-dependent manner. Lunasin extracted from liver of rats fed with lunasin-enriched wheat (LEW) also inhibited histone acetylation confirming that the peptide is intact and bioactive. The amounts of lunasin in the developing seeds and in the rat liver correlated extremely well with the extent of inhibition of core histone acetylation.
芦那辛是一种独特的由43个氨基酸组成的癌症预防肽，最初在大豆和大麦中被报道，并且已显示出在哺乳动物细胞以及皮肤癌小鼠模型中对癌基因和化学致癌物具有化学预防作用。我们在此报告来自小麦（该肽的新来源）的芦那辛对核心组蛋白H3和H4乙酰化的抑制特性，以及用富含芦那辛的小麦（LEW）喂养大鼠后，从小鼠肝脏中检测其生物利用度。采用非放射性组蛋白乙酰转移酶测定法来测量核心组蛋白乙酰化的抑制情况。通过蛋白质免疫印迹法确定小麦中芦那辛的存在，并通过液相色谱电喷雾电离质谱法（LC-ESI-MS）进行鉴定。从不同发育阶段的小麦种子中分离出的芦那辛以剂量依赖性方式抑制核心组蛋白H3和H4的乙酰化。从用富含芦那辛的小麦（LEW）喂养的大鼠肝脏中提取的芦那辛也抑制组蛋白乙酰化，证实该肽完整且具有生物活性。发育中的种子和大鼠肝脏中芦那辛的含量与核心组蛋白乙酰化的抑制程度高度相关。
