Hosokawa Y, Nakanishi T, Yamaguchi D, Nakae H, Matsuo T
Department of Conservative Dentistry, Tokushima University School of Dentistry, Tokushima, Japan.
Clin Exp Immunol. 2005 Mar;139(3):506-12. doi: 10.1111/j.1365-2249.2005.02675.x.
The regulatory role of chemokines and chemokine receptors on specific leucocyte recruitment into periodontal diseased tissue is poorly characterized. We observed that leucocytes infiltrating inflamed gingival tissue expressed marked levels of CX3CR1. In periodontal diseased tissue, the expression of fractalkine and CX3CR1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) and further, fractalkine was distributed mainly on endothelial cells, as shown by immunohistochemistry. Moreover, we can detect CX3CR1-expressing cells infiltrated in periodontal diseased tissue by immunohistochemical staining. Furthermore, fractalkine production by human umbilical vein endothelial cells (HUVEC) was up-regulated by pathogen-associated molecular patterns (PAMPs), including Porphyromonas gingivalis lipopolysaccharide (LPS). Thus, these findings suggested that CX3CR1 and the corresponding chemokine, fractalkine may have an important regulatory role on specific leucocyte migration into inflamed periodontal tissue.
趋化因子和趋化因子受体对特定白细胞募集到牙周病组织中的调节作用目前还不太清楚。我们观察到，浸润在发炎牙龈组织中的白细胞表达了高水平的CX3CR1。在牙周病组织中，通过逆转录聚合酶链反应（RT-PCR）检测到fractalkine和CX3CR1 mRNA的表达，此外，免疫组织化学显示，fractalkine主要分布在内皮细胞上。此外，通过免疫组织化学染色，我们可以检测到浸润在牙周病组织中的CX3CR1表达细胞。此外，人脐静脉内皮细胞（HUVEC）产生的fractalkine被包括牙龈卟啉单胞菌脂多糖（LPS）在内的病原体相关分子模式（PAMP）上调。因此，这些发现表明，CX3CR1和相应的趋化因子fractalkine可能对特定白细胞迁移到发炎的牙周组织中具有重要的调节作用。
