牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞中RANTES和fractalkine表达的影响
[Effects of Porphyromnonas gingivalis lipopolysaccharide on the expression of RANTES and fractalkine in human urnbilical vein endothelial cells].
作者信息
Qi Xiaoling, Zhao Lei, Chen Shanshan, Meng Shu, Wu Yafei
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Apr;34(2):194-9. doi: 10.7518/hxkq.2016.02.018.
OBJECTIVE
A study was conducted to investigate the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the expression of regulated upon activation normal T-cell expressed and secreted (RANTES) and fractalkine in human umbilical vein endothelial cells (HUVECs).
METHODS
HUVECs were incubated with different concentrations of Pg-LPS (200, 500, and 1000 ng x mL(-1)) for 1, 6, 12, and 24 h, respectively. Then real time quantitative polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent method (ELISA) were adopted to detect the protein levels and mRNA levels of RANTES and fractalkine.
RESULTS
The RANTES protein levels and mRNA levels, as well as fractalkine mRNA levels, were significantly higher in all experimental groups of 1, 6, and 12 h than in the control group (P<0.05), except the expression of RANTES mRNA in 200 ng x mL(-1) group of 12 h and RANTES protein in 200 ng x mL(-1) group of 1 h. The expression levels of RANTES mRNA and fractalkine mRNA were highest in 1000 ng x mL(-1) group of 6 h and were 4.88- and 6.20-fold higher, respectively, than those in the control group. The expression levels of RANTES protein, mRNA, and fractalkine mRNA decreased 6 h after stimulation, and were significantly higher than those in the control group (P<0.05) in the RANTES and fractalkine in HUVEC, and such expression is important in the development of atherosclerosis 500 ng x mL(-1) group of 24 h. There was a significant difference between the expression of fractalkine mRNA in 1000 ng x mL(-1) group of 6 and 12 h than in the control group (P<0.05).
CONCLUSION
Pg-LPS infection might up-regulate the expression of RANTES and fractalkine in HUVEC, and such expression is important in the development of atherosclerosis.
目的
开展一项研究以调查牙龈卟啉单胞菌脂多糖（Pg-LPS）对人脐静脉内皮细胞（HUVECs）中活化正常T细胞表达和分泌调节因子（RANTES）及趋化因子表达的影响。
方法
将HUVECs分别与不同浓度（200、500和1000 ng·mL⁻¹）的Pg-LPS孵育1、6、12和24小时。然后采用实时定量聚合酶链反应（RT-PCR）和酶联免疫吸附法（ELISA）检测RANTES和趋化因子的蛋白水平及mRNA水平。
结果
1、6和12小时的所有实验组中，RANTES蛋白水平和mRNA水平以及趋化因子mRNA水平均显著高于对照组（P<0.05），但12小时200 ng·mL⁻¹组的RANTES mRNA表达和1小时200 ng·mL⁻¹组的RANTES蛋白除外。RANTES mRNA和趋化因子mRNA的表达水平在6小时1000 ng·mL⁻¹组中最高，分别比对照组高4.88倍和6.20倍。刺激6小时后RANTES蛋白、mRNA和趋化因子mRNA的表达水平下降，在24小时500 ng·mL⁻¹组中，HUVECs中RANTES和趋化因子的表达仍显著高于对照组（P<0.05），且这种表达在动脉粥样硬化发展中很重要。1000 ng·mL⁻¹组6小时和12小时趋化因子mRNA的表达与对照组相比存在显著差异（P<0.05）。
结论
Pg-LPS感染可能上调HUVECs中RANTES和趋化因子的表达，且这种表达在动脉粥样硬化发展中很重要。
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