利用绿色荧光蛋白和逆转录-聚合酶链反应监测播散性念珠菌病小鼠模型中白色念珠菌凝集素样序列基因的表达。
Use of green fluorescent protein and reverse transcription-PCR to monitor Candida albicans agglutinin-like sequence gene expression in a murine model of disseminated candidiasis.
作者信息
Green Clayton B, Zhao Xiaomin, Hoyer Lois L
机构信息
Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 61802, USA.
出版信息
Infect Immun. 2005 Mar;73(3):1852-5. doi: 10.1128/IAI.73.3.1852-1855.2005.
Abstract
Candida albicans PALS-green fluorescent protein (GFP) reporter strains were inoculated into mice in a disseminated candidiasis model, and GFP production was monitored by immunohistochemistry and reverse transcription-PCR (RT-PCR). GFP production from the ALS1 and ALS3 promoters was detected immunohistochemically. ALS1, ALS2, ALS3, ALS4, and ALS9 transcription was detected by RT-PCR, further identifying ALS genes expressed in this model.
摘要
将白色念珠菌PALS-绿色荧光蛋白（GFP）报告菌株接种到播散性念珠菌病模型的小鼠体内，通过免疫组织化学和逆转录-聚合酶链反应（RT-PCR）监测GFP的产生。通过免疫组织化学检测到来自ALS1和ALS3启动子的GFP产生。通过RT-PCR检测ALS1、ALS2、ALS3、ALS4和ALS9的转录，进一步鉴定在该模型中表达的ALS基因。
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