Rizk Nashwa M H, Othman Abdel-Hameed M
Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Sadat City, Egypt.
Anal Sci. 2005 Feb;21(2):107-10. doi: 10.2116/analsci.21.107.
Novel miniaturized polyurethane (PU) membrane sensors in an all-solid state graphite support were developed, electrochemically evaluated and used for the assay of thiopental drug. The thiopental (T) sensors are based on the formation of ion-association complexes of thiopental with copper(II) and cobalt(II)-bathophenanthroline (bphen) counter anions as electroactive materials dispersed in a polyurethane matrix. The sensors show a linear response for thiopental over the range of 1 x 10(-1) - 5 x 10(-5) M thiopental at 25 degrees C over the pH range 6 - 11 with anionic slopes of -28.7 and -28.3 mV decade(-1) with Cu- and Co-bphen thiopental membrane sensors, respectively. These sensors exhibit a fast response time (25 - 45 s), a low detection limit (5 x 10(-6) M), a long lifetime (7 weeks) and good stability. The selectivity coefficients for thiopental sensors relative to the number of interfering anions, were investigated. These sensors were used for the direct potentiometry of thiopental in a pharmaceutical formulation and human serum. Results with mean accuracy of 99.8 +/- 0.5% of nominal were obtained, which compare well with data obtained using spectrophotometric (UV-Vis) and British Pharmacopoeia (BP) methods.
开发了一种新型的全固态石墨支撑的小型化聚氨酯（PU）膜传感器，对其进行了电化学评估，并用于硫喷妥药物的测定。硫喷妥（T）传感器基于硫喷妥与铜（II）和钴（II）-二氮杂菲（bphen）抗衡阴离子形成离子缔合络合物，这些抗衡阴离子作为电活性材料分散在聚氨酯基质中。在25℃、pH值为6 - 11的条件下，铜和钴-二氮杂菲硫喷妥膜传感器对硫喷妥的线性响应范围为1×10⁻¹ - 5×10⁻⁵ M，阴离子斜率分别为-28.7和-28.3 mV/decade⁻¹。这些传感器具有快速响应时间（25 - 45秒）、低检测限（5×10⁻⁶ M）、长寿命（七周）和良好的稳定性。研究了硫喷妥传感器相对于干扰阴离子数量的选择性系数。这些传感器用于药物制剂和人血清中硫喷妥的直接电位测定。获得的结果平均准确度为标称值的99.8±0.5%，与使用分光光度法（紫外-可见）和英国药典（BP）方法获得的数据相比良好。
