Vassallo José, Camargo Leandro Azevedo de, Chagas Cristiano Aparecido, Pinto Glauce Aparecida, Endo Luiza Hayashi
Department of Anatomical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), P.O. Box 6111, CEP 13083-970, Campinas, SP, Brazil.
Int J Pediatr Otorhinolaryngol. 2005 Mar;69(3):345-9. doi: 10.1016/j.ijporl.2004.10.006. Epub 2004 Dec 1.
Herpes simplex virus (HSV) has been described as cause of acute tonsillitis. It has also been found in nasopharyngeal florid lymphoid infiltrate, mostly composed of CD4+, CD56+ T-cells, simulating lymphoma. In spite of its widespread prevalence in latent form, to the best of our knowledge no study is available on in situ detection of HSV in chronically hyperplastic nasopharyngeal lymphoid tissue. The purpose of the present study was to search for the presence of HSV 1 and 2 in 21 adenoids and 15 tonsils from children (2-12 years of age) in which these organs had been surgically removed due to hypertrophy.
Paraffin wax-embedded sections from the 36 cases were submitted to the in situ hybridization technique, using the biotinilated probe to Herpes simplex virus 1 and 2 (Pan Path, Amsterdam) and the Rembrandt Universal DISH & HRP Detection Kit (Pan Path, Amsterdam). Positive control consisted of a previously tested Herpes infected lung.
In none of the 36 cases studied were positive nuclei detected in adenoid and tonsils, either in lymphoid, in stroma or in epithelial cells, as those seen in the positive control.
HSV does not seem to be implied in tonsil or adenoid chronic lymphoid hyperplasia. These organs do not seem to harbor the virus latently, or the amount of virus is too low to be detected without amplification methods.
单纯疱疹病毒（HSV）已被描述为急性扁桃体炎的病因。它也在鼻咽部 florid 淋巴浸润中被发现，主要由 CD4 +、CD56 + T 细胞组成，类似淋巴瘤。尽管其以潜伏形式广泛流行，但据我们所知，尚无关于慢性增生性鼻咽淋巴组织中 HSV 原位检测的研究。本研究的目的是在 21 例腺样体和 15 例扁桃体（来自 2 - 12 岁因肥大而接受手术切除的儿童）中寻找 HSV - 1 和 HSV - 2 的存在情况。
对 36 例病例的石蜡包埋切片采用原位杂交技术，使用针对单纯疱疹病毒 1 和 2 的生物素化探针（泛病理公司，阿姆斯特丹）以及伦勃朗通用 DISH & HRP 检测试剂盒（泛病理公司，阿姆斯特丹）。阳性对照为先前检测过的疱疹感染肺组织。
在研究的 36 例病例中，无论是在腺样体和扁桃体的淋巴组织、基质或上皮细胞中，均未检测到如阳性对照中所见的阳性细胞核。
HSV 似乎与扁桃体或腺样体的慢性淋巴组织增生无关。这些器官似乎不会潜伏感染该病毒，或者病毒量过低，若无扩增方法则无法检测到。
