Zhang Xiaotong, Li Hongyue, Liu Xuemei, Zhang Qing, Liu Hui, Wang Xiangling, Ling Ying
Department of Otolaryngology, the Second Teaching Hospital, Medical College of Xi'an Jiaotong University, Xi'an, 710004, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2009 Dec;23(24):1108-11.
To investigate the epidemiology of EBV in adenoidal hypertrophy and chronic tonsillitis and discuss the affection of EBV on the nosogenesis of adenoidal hypertrophy and tonsillitis of children.
Fifty-two children with chronic tonsillitis and/or adenoidal hypertrophy had the operations of the tonsillectomy and/or the adenoidectomy. These tissues resected and plasma of all cases were detected to find EBV-DNA by RQ PCR.
The infection rate of EBV in the tissues of adenoidal hypertrophy and tonsillitis of children was 51.9%. The boys' infection rate of EBV was 50.0%, and the girls' infection rate of EBV was 55.6%, which had not significantly different. The EBV infection rate in the tissues of tonsillitis was 40.4%, The EBV infection rate in the tissues of adenoidal hypertrophy was 48.9%, which had not significant difference. The school age group (7- to 14-years-old) presented higher infection rate of EBV in the tissues of adenoid and tonsil (65.5%) than the pre-school children group (2- to 6-years-old) (34.8%). Comparing the copies numbers of EBV-DNA in the different degrees of adenoidal hypertrophy, we found that the copies numbers of EBV-DNA in the severe hypertrophy group were higher than the midrange and slight hypertrophy groups (P<0.05). Meanwhile we detected EBV-DNA in these childrens' blood plasma by RQ-PCR. No blood plasma was detected EBV-DNA copies higher than normal (< 1 x 10(3) copies/ml).
The tissues of adenoidal hypertrophy and tonsillitis had same sensitivity to EBV. There was not significant difference between the infection rates of the boys and girls with adenoidal hypertrophy and/or tonsillitis. With these children growing up and the course of diseases prolonging, the infection rate of EBV increased correspondingly. There was a certain correlation between the hypertrophy of adenoid and EBV. There were no EBV-DNA fragments in blood plasma of the children with adenoidal hypertrophy and/or tonsillitis. So there were essential different between benign hyperplasia and nasopharyngeal carcinoma.
探讨EB病毒（EBV）在腺样体肥大和慢性扁桃体炎中的流行病学情况，并讨论EBV对儿童腺样体肥大和扁桃体炎发病机制的影响。
52例慢性扁桃体炎和/或腺样体肥大患儿接受扁桃体切除术和/或腺样体切除术。采用实时荧光定量聚合酶链反应（RQ PCR）检测所有病例切除的组织及血浆中的EBV-DNA。
儿童腺样体肥大和扁桃体炎组织中EBV感染率为51.9%。男孩EBV感染率为50.0%，女孩EBV感染率为55.6%，差异无统计学意义。扁桃体炎组织中EBV感染率为40.4%，腺样体肥大组织中EBV感染率为48.9%，差异无统计学意义。学龄组（7至14岁）腺样体和扁桃体组织中EBV感染率（65.5%）高于学龄前儿童组（2至6岁）（34.8%）。比较不同程度腺样体肥大中EBV-DNA拷贝数，发现重度肥大组EBV-DNA拷贝数高于中度和轻度肥大组（P<0.05）。同时采用RQ-PCR检测这些患儿血浆中的EBV-DNA，未检测到血浆中EBV-DNA拷贝数高于正常水平（<1×10³拷贝/ml）。
腺样体肥大和扁桃体炎组织对EBV的敏感性相同。腺样体肥大和/或扁桃体炎患儿的男、女感染率差异无统计学意义。随着这些儿童年龄增长和病程延长，EBV感染率相应增加。腺样体肥大与EBV之间存在一定相关性。腺样体肥大和/或扁桃体炎患儿血浆中无EBV-DNA片段。因此，良性增生与鼻咽癌有本质区别。
