Chagas Cristiano Aparecido, Endo Luiza Hayashi, Sakano Eulália, Pinto Glauce Aparecida, Brousset Pierre, Vassallo José
Department of Anatomical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), PO Box 6111, CEP 13083-970, Campinas, SP, Brazil.
Int J Pediatr Otorhinolaryngol. 2006 Jan;70(1):65-72. doi: 10.1016/j.ijporl.2005.04.030. Epub 2005 Jun 23.
Human herpesvirus 8 (HHV8) has been associated with multicentric Castleman's disease, Kaposi's sarcoma and effusion non-Hodgkin's lymphoma. Epidemiological studies have shown seropositivity in variable proportions of populations. It seems to be sexually transmitted among adults and through oral contact among children. The virus has been demonstrated in desquamating oral epithelial cells, but there is no report on its presence in the Waldeyer's ring. The purpose of the present study is to detect HHV8 in tonsils and adenoids from children up to 20 years of age in which these organs had been surgically removed due to hypertrophy, using immunohistochemistry and in situ hybridization.
Paraffin wax-embedded sections consisting of 181 tonsils and 162 adenoids from 293 patients were analyzed. HHV8 was detected by immunohistochemistry (IHC) using the anti-LNA1 antibody (Novocastra) and the LSAB+ detection system (Dako). For the in situ hybridization (ISH), the T1-1 probe for the viral mRNA and the detection system used were provided by Novocastra.
In 20 cases (6.83%), HHV8 was detected in cells morphologically characterized as lymphoid. In three of them epithelial cells were also positive. In 19 cases, the virus was detected in tonsils and in just 1 case in an adenoid. In all 20 cases detection was possible by ISH, whereas in only 2 of them there was a concomitant positivity by IHC.
Our data support the oral route of contamination by HHV8 in children, in whom tonsils and adenoids may harbor the virus. It is found especially in tonsils and only rarely in adenoids. In these organs, ISH is the method of choice to detect this virus, probably due to the small amount of viral proteins.
人类疱疹病毒8型（HHV8）与多中心Castleman病、卡波西肉瘤及体腔积液性非霍奇金淋巴瘤相关。流行病学研究表明不同人群中存在不同比例的血清阳性。该病毒似乎在成人间通过性传播，在儿童间通过口腔接触传播。已在脱屑的口腔上皮细胞中证实有该病毒存在，但尚无关于其在咽淋巴环中存在情况的报道。本研究的目的是利用免疫组织化学和原位杂交技术，检测因肥大而接受手术切除的20岁以下儿童扁桃体和腺样体中的HHV8。
分析了293例患者的181个扁桃体和162个腺样体的石蜡包埋切片。使用抗LNA1抗体（诺华卡斯）和LSAB +检测系统（达科）通过免疫组织化学（IHC）检测HHV8。对于原位杂交（ISH），用于病毒mRNA的T1 - 1探针及所用检测系统由诺华卡斯提供。
在20例（6.83%）中，在形态学上特征为淋巴细胞的细胞中检测到HHV8。其中3例上皮细胞也呈阳性。19例在扁桃体中检测到病毒，仅1例在腺样体中检测到。所有20例均可通过ISH检测到病毒，而仅2例同时通过IHC呈阳性。
我们的数据支持儿童中HHV8通过口腔途径污染，扁桃体和腺样体可能携带该病毒。尤其在扁桃体中发现，在腺样体中很少见。在这些器官中，ISH是检测该病毒的首选方法，可能是由于病毒蛋白含量较少。
