Vallochi Adriana Lima, Muccioli Cristina, Martins Maria Cristina, Silveira Cláudio, Belfort Rubens, Rizzo Luiz Vicente
Department of Immunology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
Am J Ophthalmol. 2005 Feb;139(2):350-1. doi: 10.1016/j.ajo.2004.07.040.
To determine Toxoplasma gondii genotype in scars of retinochoroiditis in humans.
Immunopathology analysis.
DNA was isolated from retinochoroidal specimens taken from the borders of the scars from eye bank eyes as well as enucleated eyes from uveitis patients with retinochoroidal toxoplasmic scars. The SAG2 region was amplified by polymerase chain reaction and analyzed by restriction fragment length polymorphism.
Of the 92 eyes studied, 9 had toxoplasmic scars. All of them, as well as the 2 enucleated eyes, disclosed the presence of type I parasites by the gel electrophoresis analysis of the restriction fragment length polymorphism from SAG2.
These results suggest that type I strain seems to be responsible for the ocular infections in the population studied.
确定人类视网膜脉络膜炎瘢痕中的弓形虫基因型。
免疫病理学分析。
从眼库眼的瘢痕边缘以及患有视网膜脉络膜弓形虫瘢痕的葡萄膜炎患者的摘除眼中获取视网膜脉络膜标本，提取DNA。通过聚合酶链反应扩增SAG2区域，并进行限制性片段长度多态性分析。
在研究的92只眼中，9只存在弓形虫瘢痕。通过对SAG2限制性片段长度多态性进行凝胶电泳分析，所有这些眼以及2只摘除眼均显示存在I型寄生虫。
这些结果表明，I型菌株似乎是所研究人群眼部感染的病因。
