Costa Deise F, Nascimento Heloisa, Sutili Aline, Nobrega Fernando A J, Fowler Flavio, Nobrega Mario Junqueira, Garrido Cristina, de Oliveira Dias Janaina, Adán Consuelo B D, Rizzo Luiz Vicente, Silveira Claudio, Belfort Rubens, Commodaro Alessandra G
Department of Ophthalmology, Federal University of Sao Paulo and Vision Institute, Rua Botucatu, 821, São Paulo, SP, 04023-062, Brazil.
Eye Bank of Joinville, Joinville, SC, Brazil.
Parasitol Res. 2017 Jul;116(7):2031-2033. doi: 10.1007/s00436-017-5474-2. Epub 2017 May 15.
Ocular toxoplasmosis is the main cause of posterior uveitis worldwide frequently leading to vision loss. In Brazil, the seroprevalence of Toxoplasma gondii infection ranges from 50 to 80% depending of the region studied. The frequency of toxoplasmic retinal scar may reach 18% of the adults in the South of Brazil. Our goal was to determine the frequency of T. gondii DNA in retinas from eye banks from different regions in Brazil. A total of 162 eyes were obtained from eye banks in Manaus (n = 60), Sao Paulo (n = 60), Chapeco (n = 26), and Joinville (n = 16). The retinas were macroscopically analyzed and collected for DNA extraction. Real-time PCR (qPCR) was performed using the T. gondii B1 marker. By qPCR, a higher frequency of T. gondii DNA in the retinas from the eye bank of Joinville (25%) was found when compared to Manaus (5%). The retinas from Sao Paulo and Chapeco were qPCR negative. Clinical examination determined the retina lesions to be compatible with toxoplasmosis in the following frequencies: Joinville (62.5%), Manaus (10%), Sao Paulo (6.7%), and Chapeco (15.4%).
眼部弓形虫病是全球后葡萄膜炎的主要病因，常导致视力丧失。在巴西，根据所研究的地区不同，弓形虫感染的血清阳性率在50%至80%之间。在巴西南部，弓形虫性视网膜瘢痕的发生率在成年人中可达18%。我们的目标是确定巴西不同地区眼库中视网膜组织弓形虫DNA的频率。总共从玛瑙斯（n = 60）、圣保罗（n = 60）、沙佩科（n = 26）和茹安维尔（n = 16）的眼库获取了162只眼睛。对视网膜进行宏观分析并收集用于DNA提取。使用弓形虫B1标记物进行实时荧光定量聚合酶链反应（qPCR）。通过qPCR发现，与玛瑙斯（5%）相比，茹安维尔眼库的视网膜中弓形虫DNA的频率更高（25%）。圣保罗和沙佩科的视网膜qPCR检测为阴性。临床检查确定视网膜病变与弓形虫病相符的频率如下：茹安维尔（62.5%）、玛瑙斯（10%）、圣保罗（6.7%）和沙佩科（15.4%）。
