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接种疫苗后发生速发型过敏反应的犬只对疫苗成分的IgE反应性。

IgE reactivity to vaccine components in dogs that developed immediate-type allergic reactions after vaccination.

作者信息

Ohmori Keitaro, Masuda Kenichi, Maeda Sadatoshi, Kaburagi Yukiko, Kurata Keigo, Ohno Koichi, Deboer Douglas J, Tsujimoto Hajime, Sakaguchi Masahiro

机构信息

Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

出版信息

Vet Immunol Immunopathol. 2005 Apr 8;104(3-4):249-56. doi: 10.1016/j.vetimm.2004.12.003.

DOI:10.1016/j.vetimm.2004.12.003

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7112531/

Abstract

Allergic reactions after vaccination are considered as an important practical problem in dogs; however, their immunological mechanism has not been well understood. The present study was designed to investigate the relationship between IgE reactivity to the vaccines and immediate-type allergic reactions after vaccination in dogs. Sera from 10 dogs that developed immediate-type allergic reactions such as circulatory collapse, cyanosis, dyspnea, facial edema, and vomiting within 1h after vaccination with non-rabies monovalent or combined vaccines and sera from 50 dogs that did not develop allergic reactions after vaccination were collected. Serum IgE reactivity to the injected vaccines was measured by fluorometric ELISA using a mouse monoclonal anti-dog IgE antibody. Then, IgE reactivity to fetal calf serum (FCS) and stabilizer proteins (gelatin, casein, and peptone) included in the vaccines was measured in sera that had high levels of IgE to the vaccines. Levels of serum specific IgE to the vaccines in dogs with immediate-type allergic reactions (59-4173 fluorescence units [FU], mean +/- S.D.: 992.5 +/- 1181.9 FU) were significantly higher than those in control dogs (38-192 FU, 92.4 +/- 43.3 FU) (P < 0.001). Of the eight dogs that developed immediate-type allergic reactions and had high levels of serum specific IgE to the vaccines, seven had specific IgE directed to FCS. The IgE reactivity to the vaccines in sera from these dogs was almost completely inhibited by FCS. The other one dog had serum IgE directed to gelatin and casein included in the vaccine as stabilizers. The results obtained in this study suggest that immediate-type allergic reactions after vaccination in dogs were induced by type I hypersensitivity mediated by IgE directed to vaccine components. In addition, FCS, gelatin, and casein included in vaccines could be the causative allergens that induced immediate-type allergic reactions after vaccination in dogs.

摘要

疫苗接种后的过敏反应被认为是犬类中一个重要的实际问题；然而，其免疫机制尚未得到很好的理解。本研究旨在调查犬类中针对疫苗的IgE反应性与疫苗接种后速发型过敏反应之间的关系。收集了10只犬在接种非狂犬病单价或联合疫苗后1小时内出现速发型过敏反应（如循环衰竭、发绀、呼吸困难、面部水肿和呕吐）的血清，以及50只接种疫苗后未出现过敏反应的犬的血清。使用小鼠单克隆抗犬IgE抗体通过荧光酶联免疫吸附测定法（fluorometric ELISA）测量血清对注射疫苗的IgE反应性。然后，在对疫苗具有高水平IgE的血清中测量对疫苗中包含的胎牛血清（FCS）和稳定剂蛋白（明胶、酪蛋白和蛋白胨）的IgE反应性。出现速发型过敏反应的犬对疫苗的血清特异性IgE水平（59 - 4173荧光单位[FU]，平均值±标准差：992.5±1181.9 FU）显著高于对照犬（38 - 192 FU，92.4±43.3 FU）（P < 0.001）。在8只出现速发型过敏反应且对疫苗具有高水平血清特异性IgE的犬中，7只具有针对FCS的特异性IgE。这些犬血清中对疫苗的IgE反应性几乎完全被FCS抑制。另一只犬的血清IgE针对疫苗中作为稳定剂包含的明胶和酪蛋白。本研究获得的结果表明，犬类疫苗接种后的速发型过敏反应是由针对疫苗成分的IgE介导的I型超敏反应引起的。此外，疫苗中包含的FCS、明胶和酪蛋白可能是诱导犬类疫苗接种后速发型过敏反应的致敏原。