Shen Y, Stalf T, Mehnert C, Eichenlaub-Ritter U, Tinneberg H-R
Centre of In-Vitro-Fertilisation (CIF) in Justus-Liebig-University, D-35392 Giessen, Germany.
Hum Reprod. 2005 Jun;20(6):1596-606. doi: 10.1093/humrep/deh811. Epub 2005 Feb 25.
Failures in expression of zona proteins correlate to subfertility in animals. Low expression of the zona proteins by the growing human oocyte may indicate reduced developmental potential. Therefore, we non-invasively analysed the thickness and the structure of the zona pellucida (ZP) of human oocytes with respect to embryo fate after ICSI.
Retardance magnitude and thickness of the inner, middle and outer layers of the ZP were quantitatively analysed by a Polscope in 166 oocytes selected for transfer after ICSI (63 patients; 32.8 +/- 4.4 years) on the basis of pronuclear score at day 1. Blastomere number was determined at day 2. Data were compared between conception cycles (CC; 65 oocytes/23 patients) and non-conception cycles (NCC; 101 oocytes/40 patients) and with respect to maternal age.
The thickness was slightly elevated (P < 0.001), and the mean magnitude of light retardance was nearly 30% higher (P < 0.001) in the inner layer of the zona pellucida of oocytes contributing to CC compared to NCC. Embryos in the CC group tended to develop faster.
The magnitude of light retardance by the zona pellucida inner layer appears to present a unique non-invasive marker for oocyte developmental potential.
透明带蛋白表达异常与动物生育力低下相关。生长中的人类卵母细胞透明带蛋白低表达可能表明其发育潜能降低。因此，我们对卵胞浆内单精子注射（ICSI）后人类卵母细胞透明带（ZP）的厚度和结构进行了非侵入性分析，以了解胚胎发育情况。
利用偏振光显微镜对166枚在ICSI后根据第1天原核评分被选作移植的卵母细胞（63例患者；32.8±4.4岁）的ZP内层、中层和外层的延迟量及厚度进行定量分析。在第2天确定卵裂球数量。比较了妊娠周期（CC；65枚卵母细胞/23例患者）和非妊娠周期（NCC；101枚卵母细胞/40例患者）的数据，并对产妇年龄进行了比较。
与NCC相比，对CC有贡献的卵母细胞透明带内层厚度略有增加（P<0.001），平均光延迟量高出近30%（P<0.001）。CC组的胚胎发育往往更快。
透明带内层的光延迟量似乎是卵母细胞发育潜能的一个独特的非侵入性标志物。
