Scalarone G M, Legendre A M, Clark K A, Pusater K
Department of Biological Sciences, Idaho State University, Pocatello 83209.
J Med Vet Mycol. 1992;30(1):43-9. doi: 10.1080/02681219280000061.
A DNA probe assay (AccuProbe, Gen-Probe, Inc., San Diego, CA) for culture confirmation of Blastomyces dermatitidis in clinical isolates was evaluated using 72 isolates of B. dermatitidis (62 dog isolates, five from other animals, four human isolates and one soil isolate). In addition, specificity determinations were performed using 28 American Type Culture Collection (ATCC) fungal isolates, other than B. dermatitidis, representing 21 different genera. The non-isotopic hybrid protection assay, that can be performed in less than 1 h, uses a chemiluminescent acridinium ester DNA probe that is homologous to ribosomal RNA sequences of B. dermatitidis. Positive results were obtained with all 72 B. dermatitidis isolates (100% sensitivity). In addition, no positive results were evidenced with the other 28 non-B. dermatitidis fungal isolates, including six strains of Histoplasma capsulatum (100% specificity).
使用72株皮炎芽生菌（62株犬分离株、5株其他动物分离株、4株人类分离株和1株土壤分离株）对一种用于临床分离株中皮炎芽生菌培养确认的DNA探针检测法（AccuProbe，Gen-Probe公司，加利福尼亚州圣地亚哥）进行了评估。此外，使用28株美国典型培养物保藏中心（ATCC）的真菌分离株（除皮炎芽生菌外）进行特异性测定，这些分离株代表21个不同的属。这种非同位素杂交保护检测法可在不到1小时内完成，它使用一种与皮炎芽生菌核糖体RNA序列同源的化学发光吖啶酯DNA探针。所有72株皮炎芽生菌分离株均获得阳性结果（敏感性100%）。此外，其他28株非皮炎芽生菌真菌分离株，包括6株荚膜组织胞浆菌，均未出现阳性结果（特异性100%）。
