Escoula L
Station de Pharmacologie-Toxicologie, I.N.R.A., Toulouse, France.
J Environ Pathol Toxicol Oncol. 1992 Mar-Apr;11(2):45-8.
The maximum patulin production by Penicillium granulatum was obtained during the 16th day of culture at 26 degrees C (2.9 g/L) on liquid Czapek medium (+ 8 p. 1000 glucose), followed by 32 degrees C after 24 days and 20 degrees C after 32 days. At 26 degrees C patulin production was maximum before the greatest rate of sporulation when the pH of the medium was about 4. Toxin production was studied by anaerobic culture for 75 days and then the cultures were exposed to ambient air. P. granulatum did not produce its toxin under anaerobic conditions. An in vitro fermentation model with rumen liquor was used, and within 4 hr acetic acid production declined and dose-dependent protein synthesis was inhibited with 20, 100, and 300 microgram patulin/mL rumen liquid.
颗粒青霉在26℃的液体察氏培养基（+8‰葡萄糖）上培养16天时，展青霉素产量最高（2.9克/升），24天后为32℃，32天后为20℃。在26℃时，当培养基pH值约为4且孢子形成速率最高之前，展青霉素产量最高。通过厌氧培养75天研究毒素产生情况，然后将培养物暴露于环境空气中。颗粒青霉在厌氧条件下不产生毒素。使用瘤胃液建立体外发酵模型，在4小时内，乙酸产量下降，且当瘤胃液中展青霉素浓度为20、100和300微克/毫升时，剂量依赖性蛋白质合成受到抑制。
