Kun Gao, Wan Mingxi
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
Ultrasonics. 2005 Mar;43(5):351-5. doi: 10.1016/j.ultras.2004.08.002.
The aim of this study was to investigate the agreement in HIFU-induced lesion sizes between measurements based on gross histological examination and those from images. Experiments were conducted in an experimental arrangement with a three-way multiscan ultrasonic inspection system and imaging was done by B-mode ultrasound (US). Bovine thigh muscle with and without fascia lata was treated with an in situ spatially averaged focal intensity ranging between 750 W cm(-2) and 1565 W cm(-2) and sonication-time was variable from 8 to 20s. Assessment of the two measurement methods showed a rather weak correlation in the samples without fascia lata. For clarity and convenience in the discussion, sample muscles with and without fascia lata were labeled F and M, respectively. It was difficult to measure the lesion size from ultrasonographic images of F samples, so there was disagreement in the samples with fascia lata. This investigation showed that the presence of fascia lata in bovine thigh muscle has the likely effect of affecting the ultrasound image and makes it difficult to distinguish coagulated tissue from surrounding healthy tissue. There was no significant correlation between ultrasonography and the gross histological findings in M samples. Data supported that at for an in situ spatially averaged focal intensity ranging between 750 W cm(-2) and 1565 W cm(-2) and relatively shorter exposures (sonication-time variable from 1 to 8s) higher correlation between image and gross histology measurement was found in excised bovine muscle specimens.
本研究的目的是调查基于大体组织学检查的高强度聚焦超声（HIFU）诱导损伤大小测量值与图像测量值之间的一致性。实验采用具有三向多扫描超声检测系统的实验装置进行，成像通过B型超声（US）完成。对带有和不带有阔筋膜的牛大腿肌肉进行治疗，原位空间平均聚焦强度在750 W/cm²至1565 W/cm²之间，超声处理时间在8至20秒之间变化。对两种测量方法的评估表明，在没有阔筋膜的样本中，相关性相当弱。为便于讨论，将带有和不带有阔筋膜的样本肌肉分别标记为F和M。从F样本的超声图像中很难测量损伤大小，因此在带有阔筋膜的样本中存在差异。本研究表明，牛大腿肌肉中阔筋膜的存在可能会影响超声图像，使其难以区分凝固组织与周围健康组织。在M样本中，超声检查与大体组织学结果之间没有显著相关性。数据支持，对于原位空间平均聚焦强度在750 W/cm²至1565 W/cm²之间且暴露时间相对较短（超声处理时间在1至8秒之间变化）的情况，在切除的牛肌肉标本中发现图像与大体组织学测量之间具有更高的相关性。
