Tan Xiao-Hui, Zhao Min, Pan Kai-Feng, Dong Ying, Dong Bin, Feng Gui-Jian, Jia Guang, Lu You-Yong
Beijing Molecular Oncology Laboratory, Beijing Institute for Cancer Research, School of Oncology, Peking University, Da-Hong-Luo-Chang Street 1#, Western District, Beijing 100034, People's Republic of China.
Cancer Lett. 2005 Mar 18;220(1):101-14. doi: 10.1016/j.canlet.2004.07.049.
To explore whether DNA polymerase beta (pol beta) contributes to the malignant transformation of gastric mucosa, we examined pol beta in gastric tumor cell lines, primary tumors and precancerous lesions. Point mutations of pol beta were detected in 6 of 13 cell lines and 23 of 104 tissues including 35.0% (14/40) of gastric cancer (GC), 30.0% (3/10) of dysplasia (Dys), 28.6% (4/14) of intestinal metaplasia (IM) and 10.5% (2/19) of chronic atrophic gastritis (CAG), respectively. A frequent mutation was a T to C transition at nucleotide 889, which was observed in 4 GC cell lines, 7 GC, 2 Dys, and 2 IM. The level of pol beta expression in tumors was higher than that of their matched normal tissues and gradual changes from GC, Dys, CAG to IM. These results indicate that the mutation and overexpression of pol beta may influence the progression during gastric carcinogenesis.
