Manni Luigi, Antonelli Alessia, Costa Nicola, Aloe Luigi
Institute of Neurobiology and Molecular Medicine, Section of Neurobiology, CNR, Roma, Italy.
Basic Res Cardiol. 2005 Mar;100(2):121-30. doi: 10.1007/s00395-004-0502-7. Epub 2004 Nov 23.
The aim of this study was to investigate the role of stress and nerve growth factor (NGF) on the expression of vascular-endothelial growth factor (VEGF) and NGF high-affinity receptor (tyrosine kinase A, TrKA) in the ascending and abdominal aorta.Adult male rats were exposed to immobilization stress for one hour or injected with purified murine NGF. Four hours after treatments, rats were sacrificed and VEGF, NGF and TrkA expression in ascending and abdominal aorta evaluated. The effects of anti-NGF treatment on arterial VEGF expression and on stress-induced arterial cell proliferation were also studied in control and stressed rats.The data indicated that both stress and NGF injection induced a rapid increase of arterial VEGF associated with an elevated level of NGF and TrkA in arterial tissues. Blocking NGF action by neutralizing NGF-antibodies, results in down-regulation of stress-induced VEGF expression by arteries and in the blockade of stress-induced proliferation of cells from the arterial wall.Overall our data demonstrated that NGF is involved in the regulation of VEGF and in cardiac vessels response after emotional stress.
本研究旨在探讨应激和神经生长因子（NGF）对升主动脉和腹主动脉中血管内皮生长因子（VEGF）及NGF高亲和力受体（酪氨酸激酶A，TrKA）表达的作用。成年雄性大鼠接受1小时的固定应激或注射纯化的小鼠NGF。处理4小时后，处死大鼠并评估升主动脉和腹主动脉中VEGF、NGF和TrkA的表达。还在对照大鼠和应激大鼠中研究了抗NGF处理对动脉VEGF表达及应激诱导的动脉细胞增殖的影响。数据表明，应激和NGF注射均诱导动脉VEGF快速增加，同时动脉组织中NGF和TrkA水平升高。用中和性NGF抗体阻断NGF作用，导致动脉对应激诱导的VEGF表达下调，并阻断应激诱导的动脉壁细胞增殖。总体而言，我们的数据表明，NGF参与情绪应激后VEGF的调节及心脏血管反应。
