Zhao S, Wei F, Zou H, Xu X
Chinese Environmental Monitoring Center, Beijing, 100029.
Se Pu. 1997 Nov;15(6):508-11.
A reversed-phase high performance liquid chromatographic method for the determination of aniline derivatives in waste-water is described. Chromatographic conditions were: column, Hypersil BDS (200 mm x 4.0 mm i.d., 5 microm); mobile phase, methanol-water, gradient elution; flow rate, 1.0 mL/min; detection wavelength, 254 nm. Quantification was carried out based on the external standard of aniline derivatives. The pH of waste water sample was adjusted to 11-12 with dilute NaOH solution prior to extraction with CH2Cl2. The extractives concentrated in K-D concentrator and filtered with FH 0.5 microm membrane. Finally make up the volume to 1 mL and test it with reversed-phase high performance liquid chromatography. The linearity range of calibration curve used was 0.005-0.3 microg. The minimum detectable quantities of aniline derivatives varied from 1.20 ng to 3.00 ng. The average recoveries were from 51.57% to 107.92%, and the RSDs of the method were from 1.88% to 5.98%. The method is simple, rapid, and accurate.
描述了一种用于测定废水中苯胺衍生物的反相高效液相色谱法。色谱条件为：色谱柱，Hypersil BDS（200 mm×4.0 mm内径，5微米）；流动相，甲醇-水，梯度洗脱；流速，1.0 mL/分钟；检测波长，254纳米。基于苯胺衍生物的外标进行定量。废水样品在用二氯甲烷萃取前，用稀氢氧化钠溶液将其pH值调至11 - 12。萃取物在K - D浓缩器中浓缩，并用0.5微米的FH膜过滤。最后定容至1 mL并用反相高效液相色谱法进行检测。所用校准曲线的线性范围为0.005 - 0.3微克。苯胺衍生物的最低检测量在1.20纳克至3.00纳克之间。平均回收率为51.57%至107.92%，该方法的相对标准偏差为1.88%至5.98%。该方法简单、快速且准确。
