Huang Xiaolan, Xu Meiying
Guangdong Key Laboratory of Chemical Emergency Test, China National Analysis Center, Guangzhou, Guangzhou 510070, China.
Se Pu. 2005 May;23(3):296-8.
A high performance liquid chromatographic (HPLC) method was established for the simultaneous determination of three main pollutants (josamycin, theophylline and paracetamol) in pharmaceutical waste water. The three drug residues were concentrated with a SepPak C18 solid-phase extracted column, and eluted with methanol. The HPLC separation was performed on a Hypersil ODS column (200 mm x 4.6 mm i.d.) with a gradient eluting system of 0.025 mol/LKH2PO4-H3PO4 (pH 2.75)-methanol. The detection wavelengths were 230 nm for josamycin, 272 nm for theophylline, and 243 nm for paracetamol. The linear ranges of the drugs were 0.1-100 mg/L with correlation coefficients between 0.9993 and 0.9995. The recoveries were more than 93%, and relative standard deviations (n = 6) were less than 2.1%. The detection limits (S/N = 3) were less than 1.0 microg/L.
建立了一种高效液相色谱（HPLC）法，用于同时测定制药废水中的三种主要污染物（交沙霉素、茶碱和对乙酰氨基酚）。三种药物残留用SepPak C18固相萃取柱进行富集，并用甲醇洗脱。HPLC分离在Hypersil ODS柱（200 mm×4.6 mm内径）上进行，采用0.025 mol/L KH2PO4-H3PO4（pH 2.75）-甲醇梯度洗脱系统。交沙霉素的检测波长为230 nm，茶碱为272 nm，对乙酰氨基酚为243 nm。药物的线性范围为0.1-100 mg/L，相关系数在0.9993至0.9995之间。回收率大于93%，相对标准偏差（n = 6）小于2.1%。检测限（S/N = 3）小于1.0 μg/L。
