Liu Zan, Chou Guixin, Wang Zhengtao
China Pharmaceutical University, Nanjing 210038, China.
Zhongguo Zhong Yao Za Zhi. 2010 Mar;35(6):729-31.
To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata.
The analysis was carried out on a Boston Crest ODS column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL x min(-1) and the detection wavelength was at 215 nm.
The calibration curve was linear over the range of 0.0202-20.02 microg for kirenol. The correlation coefficient of the calibration curve was 0.99975. The average recovery was 99.62% with relative standard derivation (RSD) of 2.0%.
The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.
建立高效液相色谱法测定制豨莶草中奇壬醇的含量。
采用Boston Crest ODS柱（4.6 mm×250 mm，5μm），以乙腈-水为流动相进行梯度洗脱。流速为1.0 mL·min⁻¹，检测波长为215 nm。
奇壬醇在0.0202 - 20.02μg范围内线性关系良好，校准曲线的相关系数为0.99975。平均回收率为99.62%，相对标准偏差（RSD）为2.0%。
结果表明该方法简便、准确、重复性好，适用于制豨莶草中奇壬醇的含量测定。
