Wang W
Research Institute of Forest Ecology and Environment, Chinese Academy of Forestry, Beijing, 100091.
Se Pu. 1997 May;15(3):254-6.
A method for determination of taxol, 10-deacetyl-7-epitaxol, cephalomannine, 10-deacetyl taxol, baccatin III and 10-deacetylbaccatin III, is reported and their retention mechanism is also discussed. These compounds were separated well by RP-HPLC on a Resolvepak C18 column with methanol-water-phosphoric acid (60:40:0.5) as mobile phase and UV detector at 228nm. The powdered stems were extracted with 95% ethanol, refluxed for 8 hrs and filtered and then the residue was partitioned three times between water and chloroform. The organic phase was evaporated to dryness and the residue dissolved in reagent methanol and was submitted to HPLC analysis. The results showed that there is a linear relationship between the peak area and the amount of these compounds over the range of 0.1-2.0microg. The lowest detectable concentration of these compounds was below 0.1microg.
报道了一种测定紫杉醇、10-去乙酰基-7-表紫杉醇、三尖杉宁碱、10-去乙酰基紫杉醇、浆果赤霉素III和10-去乙酰基浆果赤霉素III的方法，并讨论了它们的保留机制。这些化合物在Resolvepak C18柱上采用反相高效液相色谱法，以甲醇-水-磷酸（60:40:0.5）为流动相，在228nm处用紫外检测器进行分离，分离效果良好。将粉末状茎用95%乙醇提取，回流8小时后过滤，然后将残渣在水和氯仿之间分配三次。将有机相蒸发至干，残渣溶于甲醇试剂中，进行高效液相色谱分析。结果表明，在0.1-2.0微克范围内，这些化合物的峰面积与含量之间存在线性关系。这些化合物的最低检测浓度低于0.1微克。
