Li Xiaoxian, Xiong Yaokang, Yu Chenhuan, Zhang Chunchun
School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Se Pu. 2010 Nov;28(11):1067-70.
A high performance liquid chromatographic (HPLC) fingerprints method was developed for the analysis of the chloroform extracts of Taxus wallichiana. The extracts were separated on an Eurospher 100 C18 column (250 mm x 4 mm, 5 microm) with the gradient elution of methanol and water at a flow rate of 1 mL/min. The detection wavelength was set at 232 nm, and the column temperature was 30 degrees C. Under the same conditions, 10 batches of the chloroform extracts of Taxus wallichiana from different habitats were analyzed with the reference substance of 10-deacetyl baccatin III (10-DAB III). The 11 peaks were selected as the characteristic peaks, and the method of principal component analysis (PCA) was applied to analyze the fingerprints of the chloroform extracts of Taxus wallichiana. The results showed that there was an interrelated relationship between habitat and quality of Taxus wallichiana. The method has good repeatability and stability, and can be utilized as an approach for the quality control of Taxus wallichiana.
建立了一种高效液相色谱（HPLC）指纹图谱法用于分析西藏红豆杉氯仿提取物。提取物在Eurospher 100 C18柱（250 mm×4 mm，5μm）上分离，以甲醇和水梯度洗脱，流速为1 mL/min。检测波长设定为232 nm，柱温为30℃。在相同条件下，用10 - 去乙酰巴卡亭III（10 - DAB III）对照品对10批不同产地的西藏红豆杉氯仿提取物进行分析。选取11个峰作为特征峰，采用主成分分析（PCA）方法对西藏红豆杉氯仿提取物指纹图谱进行分析。结果表明，西藏红豆杉的产地与质量之间存在相关性。该方法具有良好的重复性和稳定性，可作为西藏红豆杉质量控制的一种方法。
