Wang C, Yan S, Zeng L, Su J
Zhongshan University, Guangzhou, 510275.
Se Pu. 1997 Sep;15(5):396-9.
The alga Sargassum carpophyllum J. Agardh collected from the South China Sea was immersed in 95% ethyl alcohol. The combined extract was partitioned between ethyl acetate and water. The ethyl acetate phase was subjected to pressure-reduced chromatography over Silica H, eluted with a step gradient of EtOAc in petroleum ether. Two main fractions of sterols (I) and fatty acids (II) were obtained. The composition of I and II were identified by GC/MS. The fraction Ia was obtained, as a main composition of I, by repeated flash chromatography. On the basis of spectroscopic analysis, Ia was identified to be fucosterol.
将采自中国南海的叶托马尾藻浸泡于95%乙醇中。合并提取物用乙酸乙酯和水进行分配。乙酸乙酯相在硅胶H上进行减压色谱分离，用乙酸乙酯在石油醚中的梯度洗脱。得到甾醇（I）和脂肪酸（II）两个主要馏分。通过气相色谱/质谱联用仪鉴定了I和II的组成。通过反复快速色谱法得到馏分Ia，它是I的主要成分。基于光谱分析，鉴定Ia为岩藻甾醇。
