Kordali Saban, Cakir Ahmet, Mavi Ahmet, Kilic Hamdullah, Yildirim Ali
Department of Plant Protection, Faculty of Agriculture, Kazim Karabekir Education Faculty, Ataturk University, 25240 Erzurum, Turkey.
J Agric Food Chem. 2005 Mar 9;53(5):1408-16. doi: 10.1021/jf048429n.
The compositions of essential oils isolated from the aerial parts of Artemisia absinthium, Artemisia santonicum, and Artemisia spicigera by hydrodistillation were analyzed by GC-MS, and a total of 204 components were identified. The major components of these essential oils were camphor (34.9-1.4%), 1,8-cineole (9.5-1.5%), chamazulene (17.8-nd%), nuciferol propionate (5.1-nd%), nuciferol butanoate (8.2-nd%), caryophyllene oxide (4.3-1.7%), borneol (5.1-0.6%), alpha-terpineol (4.1-1.6%), spathulenol (3.7-1.3%), cubenol (4.2-0.1%), beta-eudesmol (7.2-0.6%), and terpinen-4-ol (3.5-1.2%). The antifungal activities of these essential oils were tested against 11 plant fungi and were compared with that of a commercial antifungal reagent, benomyl. The results showed that all of the oils have potent inhibitory effects at very broad spectrum against all of the tested fungi. Pure camphor and 1,8-cineole, which are the major components of the oils, were also tested for antifungal activity against the same fungal species. Unlike essential oils, these pure compounds were able to show antifungal activity against only some of the fungal species. In addition, the antioxidant and DPPH radical scavenging activities of the essential oils, camphor, and 1,8-cineole were determined in vitro. All of the studied essential oils showed antioxidant activity, but camphor and 1,8-cineole did not.
采用气相色谱 - 质谱联用(GC - MS)分析法,对通过水蒸馏法从苦艾、山道年蒿和香蒿地上部分分离得到的挥发油成分进行了分析,共鉴定出204种成分。这些挥发油的主要成分有樟脑(34.9 - 1.4%)、1,8 - 桉叶素(9.5 - 1.5%)、天蓝烃(17.8 - 未检出)、丙酸核叶油醇酯(5.1 - 未检出)、丁酸核叶油醇酯(8.2 - 未检出)、氧化石竹烯(4.3 - 1.7%)、冰片(5.1 - 0.6%)、α - 松油醇(4.1 - 1.6%)、匙叶桉油烯醇(3.7 - 1.3%)、库贝醇(4.2 - 0.1%)、β - 桉叶醇(7.2 - 0.6%)和萜品 - 4 - 醇(3.5 - 1.2%)。测试了这些挥发油对11种植物真菌的抗真菌活性,并与商业抗真菌试剂苯菌灵进行了比较。结果表明,所有挥发油对所有测试真菌均具有非常广谱的强效抑制作用。还对挥发油的主要成分纯樟脑和1,8 - 桉叶素针对相同真菌种类进行了抗真菌活性测试。与挥发油不同,这些纯化合物仅对部分真菌种类表现出抗真菌活性。此外,还在体外测定了挥发油、樟脑和1,8 - 桉叶素的抗氧化及清除DPPH自由基活性。所有研究的挥发油均表现出抗氧化活性,但樟脑和1,8 - 桉叶素未表现出抗氧化活性。