Limam Inès, Ghali Ridha, Abdelkarim Mohamed, Ouni Anis, Araoud Manel, Abdelkarim Mouaadh, Hedhili Abderrazek, Ben-Aissa Fennira Fatma
PRF of Onco-Hematology, Faculty of medicine of Tunis, Tunis El Manar University, Tunis, Tunisia.
Human genetics laboratory, Faculty of medicine of Tunis, Tunis El Manar University, Tunis, Tunisia.
Plant Methods. 2024 May 2;20(1):59. doi: 10.1186/s13007-024-01185-4.
Artemisia campestris L. (AC) leaves are widely recognized for their importance in traditional medicine. Despite the considerable amount of research conducted on this plant overworld, the chemical composition and the biological activity of the leaves grown in Tunisia remains poorly investigated. In this study of AC, a successive extraction method was employed (hexane, ethyl acetate and methanol) to investigate its bioactive constituents by LC-MS analysis, and their antioxidant, antibacterial, antifungal, and anticancer activities.
Data analysis revealed diverse compound profiles in AC extracts. Methanolic and ethyl acetate extracts exhibited higher polyphenolic content and antioxidant activities, while Hexane showed superior phytosterol extraction. Ethyl acetate extract displayed potent antibacterial activity against multi-resistant Staphylococcus aureus and Pseudomonas aeruginosa. Additionally, all extracts demonstrated, for the first time, robust antifungal efficacy against Aspergillus flavus and Aspergillus niger. Cytotoxicity assays revealed the significant impact of methanolic and ethyl acetate extracts on metastatic breast cancer and multiple myeloma, examined for the first time in our study. Moreover, further analysis on multiple myeloma cells highlighted that the ethyl acetate extract induced apoptotic and necrotic cell death and resulted in an S phase cell cycle blockage, underscoring its therapeutic potential.
This investigation uncovers novel findings in Tunisian AC, notably the identification of lupeol, oleanolic acid, ursolic acid, stigmasterol and β-sitosterol. The study sheds light on the promising role of AC extracts in therapeutic interventions and underscores the need for continued research to harness its full potential in medicine and pharmaceutical development.
原野蒿（Artemisia campestris L.，简称AC）叶在传统医学中的重要性已得到广泛认可。尽管全球对这种植物进行了大量研究，但对突尼斯种植的AC叶的化学成分和生物活性的研究仍很不足。在本项关于AC的研究中，采用了连续萃取法（己烷、乙酸乙酯和甲醇），通过液相色谱 - 质谱分析研究其生物活性成分及其抗氧化、抗菌、抗真菌和抗癌活性。
数据分析揭示了AC提取物中不同的化合物谱。甲醇提取物和乙酸乙酯提取物表现出较高的多酚含量和抗氧化活性，而己烷提取物的植物甾醇提取效果更佳。乙酸乙酯提取物对多重耐药金黄色葡萄球菌和铜绿假单胞菌显示出强大的抗菌活性。此外，所有提取物首次对黄曲霉和黑曲霉表现出强大的抗真菌功效。细胞毒性试验显示，甲醇提取物和乙酸乙酯提取物对转移性乳腺癌和多发性骨髓瘤有显著影响，这是我们的研究首次进行检测。此外，对多发性骨髓瘤细胞的进一步分析表明，乙酸乙酯提取物可诱导细胞凋亡和坏死性细胞死亡，并导致细胞周期阻滞在S期，凸显了其治疗潜力。
本研究揭示了突尼斯AC的新发现，特别是羽扇豆醇、齐墩果酸、熊果酸、豆甾醇和β - 谷甾醇的鉴定。该研究揭示了AC提取物在治疗干预中的潜在作用，并强调需要继续开展研究以充分挖掘其在医学和药物开发中的潜力。
