Cleary M, Shaw J M, Jenkin G, Trounson A O
Centre for Early Human Development, Monash Institute of Reproduction and Development, Monash University, Clayton, Vic, Australia.
Reprod Fertil Dev. 2004;16(7):699-707. doi: 10.1071/rd04054.
Developmentally competent oocytes can be collected from xenografted ovarian tissues; however, optimal xenograft conditions need to be established for this technique to be of use in assisted reproduction. In the present study, common wombat ovarian tissue was xenografted under the kidney capsule of nude mice to clarify the role of recipient gonadal status and donor tissue age on graft establishment, follicle development and oocyte recovery. Eighty-nine per cent of all grafts were recovered; of these, 78% contained growing follicles. In female graft recipients, follicle development to the antral stage occurred earlier in ovariectomised recipients compared with intact graft recipients. Similarly, follicle development occurred earlier in recipients of pouch young ovarian tissue grafts when compared with subadult xenografts. Follicle development proceeded to the antral stage in subadult grafts placed under the kidney capsule of male recipient mice, albeit at a slower rate than subadult grafts placed in female recipients. Oocytes were collected from grafts placed in female and male recipients, but no mature oocytes were observed at the time of collection, nor could these oocytes be matured in vitro. The present study demonstrated that common wombat pouch young tissue xenografted to female recipient mice, and subadult ovarian tissue xenografted to male recipient mice, can develop to the antral stage and can therefore facilitate oocyte collection. However, mature oocytes were not obtained using the current protocol.
发育能力良好的卵母细胞可从异种移植的卵巢组织中采集；然而，要使这项技术用于辅助生殖，需要建立最佳的异种移植条件。在本研究中，将普通袋熊的卵巢组织异种移植到裸鼠的肾包膜下，以阐明受体性腺状态和供体组织年龄对移植物建立、卵泡发育和卵母细胞回收的作用。所有移植物的回收率为89%；其中，78%含有生长中的卵泡。在雌性移植物受体中，与完整的移植物受体相比，卵巢切除受体中的卵泡发育至窦状期的时间更早。同样，与亚成年异种移植物相比，幼年袋卵巢组织移植物受体中的卵泡发育更早。置于雄性受体小鼠肾包膜下的亚成年移植物中的卵泡发育至窦状期，尽管其发育速度比置于雌性受体中的亚成年移植物慢。从置于雌性和雄性受体中的移植物中采集了卵母细胞，但在采集时未观察到成熟卵母细胞，这些卵母细胞也无法在体外成熟。本研究表明，异种移植到雌性受体小鼠的普通袋熊幼年组织以及异种移植到雄性受体小鼠的亚成年卵巢组织可发育至窦状期，因此有助于卵母细胞采集。然而，使用当前方案未获得成熟卵母细胞。
