Cleary M, Paris M C J, Shaw J, Jenkin G, Trounson A
Centre for Early Human Development, Monash Institute of Reproduction and Development, Monash University, Clayton, Victoria 3168, Australia.
Reprod Fertil Dev. 2003;15(6):333-42. doi: 10.1071/RD03063.
Ovarian tissue xenografting may be applied to increase the population size of rare or endangered animals. However, optimal grafting conditions, such as graft position and recipient hormonal status, are yet to be established. The present study, using common wombat ovarian tissue, showed that development of xenografted ovarian tissue to the antral follicle stage can be achieved irrespective of graft position. However, increased graft recovery rates and follicle survival were evident after grafting under the kidney capsule compared with grafting to subcutaneous sites. No increase in follicle development was observed after placing grafts both under the kidney capsule and subcutaneously in the one recipient compared with grafts placed under the kidney capsule alone or subcutaneously alone. Removal of the recipient's own ovaries at the time of grafting accelerated graft follicle development, with antral follicles seen by Week 12 after grafting compared with by Week 16 in recipients that retained their own ovaries. More oocytes were collected from xenograft recipients receiving hormonal stimulation before collection compared with non-stimulated recipients. No oocytes were mature (extruded a polar body) at the time of collection or after a subsequent period of in vitro maturation. This is the first study to demonstrate that antral follicle development can occur and oocytes can be collected from xenografted common wombat ovarian tissue.
卵巢组织异种移植可用于增加珍稀或濒危动物的种群数量。然而，最佳移植条件，如移植位置和受体激素状态，尚未确定。本研究使用袋熊的卵巢组织表明，无论移植位置如何，异种移植的卵巢组织都能发育到窦卵泡阶段。然而，与皮下移植相比，肾包膜下移植后移植回收率和卵泡存活率明显提高。与单独在肾包膜下或皮下移植相比，在同一受体的肾包膜下和皮下同时移植后，未观察到卵泡发育增加。移植时切除受体自身的卵巢可加速移植卵泡的发育，移植后12周可见窦卵泡，而保留自身卵巢的受体在16周时才可见。与未接受激素刺激的受体相比，在采集前接受激素刺激的异种移植受体收集到的卵母细胞更多。采集时或随后的体外成熟培养后，均未发现卵母细胞成熟（排出极体）。这是第一项证明异种移植的袋熊卵巢组织可发生窦卵泡发育并可收集卵母细胞的研究。
