Pierson J, Wang B, Neveu N, Sneek L, Côté F, Karatzas C N, Baldassarre H
Nexia Biotechnologies Inc., St Telesphore, Quebec, Canada.
Reprod Fertil Dev. 2004;16(8):795-9. doi: 10.1071/rd04066.
The present study was conducted to evaluate the follicular response and oocyte yield following repeated gonadotrophin stimulation and laparoscopic aspiration in goats and to assess the effects of the time interval between procedures and season. A total of 98 adult goats were subjected to laparoscopic ovum pick-up (LOPU) five consecutive times in a transgenic production programme. Oestrus was synchronised by means of intravaginal sponges inserted for 10 days coupled with 125 microg cloprostenol 36 h before sponge removal and LOPU, and follicular development was stimulated with 80 mg follicle stimulating hormone and 300 IU equine chorionic gonadotrophin administered 36 h before LOPU. No difference was detected in the response for LOPUs 1, 2, 3 and 4. Although a small decrease in response was detected at LOPU 5 (P < 0.05), the numbers of follicles aspirated and oocytes recovered were not different from those at LOPU 1 and LOPUs 1 and 4, respectively. With respect to time interval between LOPU and season, all intervals and seasons produced acceptable responses, with no difference in follicles aspirated and oocytes recovered between intervals and seasons. These results indicate that LOPU may be repeated up to five times in goats at different intervals and in different seasons with little or no important change in overall response.
本研究旨在评估反复进行促性腺激素刺激和腹腔镜抽吸后山羊的卵泡反应和卵母细胞产量，并评估操作间隔时间和季节的影响。在一个转基因生产项目中，总共98只成年山羊连续5次接受腹腔镜采卵（LOPU）。通过插入阴道海绵10天并在取出海绵和进行LOPU前36小时注射125微克氯前列醇来同步发情，在LOPU前36小时用80毫克促卵泡激素和300国际单位马绒毛膜促性腺激素刺激卵泡发育。在第1、2、3和4次LOPU时未检测到反应差异。虽然在第5次LOPU时检测到反应略有下降（P<0.05），但抽吸的卵泡数量和回收的卵母细胞数量分别与第1次LOPU以及第1次和第4次LOPU时没有差异。关于LOPU之间的时间间隔和季节，所有间隔和季节都产生了可接受的反应，间隔和季节之间在抽吸的卵泡和回收的卵母细胞方面没有差异。这些结果表明，在不同间隔和不同季节，山羊可以重复进行LOPU多达5次，总体反应几乎没有或没有重要变化。
