Liu Mei, Li Yong-Guo, Zhang Fang, Yang Li, Chou Gui-Xin, Wang Zheng-Tao, Hu Zhi-Bi
Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
J Sep Sci. 2007 Sep;30(14):2256-67. doi: 10.1002/jssc.200700149.
Danshen root (Salvia miltiorrhiza Radix et Rhizoma) is a representative Chinese herb containing multiple components contributing to its polyvalent bioactivities. Advanced analysis approaches are needed to obtain a comprehensive picture of the targeting constituents in complete matrix. In this study, a chromatographic fingerprinting method to monitor simultaneously the hydrophilic and lipophilic constituents was developed for the quality evaluation of Danshen root and its preparations. Ten hydrophilic and nine lipophilic components were identified using HPLC-diode array detection-electrospray-MS (HPLC-DAD-ESI/MS) by comparing with the available references and reported data. Using the established method, 13 Danshen root samples collected from different sources, and 21 batches of Danshen preparations including tablets, injections, capsules, and dropping pills produced by different manufacturers were analyzed and their chromatographic fingerprints (CFP) were constructed. The results showed that the products of Danshen roots such as the tablets and capsules contained both the hydrophilic and lipophilic components, while the injections and dropping pills contained mainly the hydrophilic components. Principal components analysis (PCA) was applied for the statistical analysis of the fingerprinting data of crude herb and its preparations. The established CFPs demonstrate the representative chemical profiling of the existing components and can be applied to the authentication and quality assessment of Danshen roots and other Danshen containing formulated preparations.
丹参（Salvia miltiorrhiza Radix et Rhizoma）是一种具有代表性的中药，含有多种成分，这些成分共同赋予其多种生物活性。需要先进的分析方法来全面了解完整基质中的目标成分。在本研究中，开发了一种同时监测亲水性和亲脂性成分的色谱指纹图谱方法，用于丹参及其制剂的质量评价。通过与现有参考文献和报道数据进行比较，使用高效液相色谱-二极管阵列检测-电喷雾质谱联用仪（HPLC-DAD-ESI/MS）鉴定了10种亲水性成分和9种亲脂性成分。采用所建立的方法，对13个不同来源的丹参样品以及21批由不同厂家生产的丹参制剂（包括片剂、注射剂、胶囊剂和滴丸剂）进行了分析，并构建了它们的色谱指纹图谱（CFP）。结果表明，丹参的片剂和胶囊剂等产品同时含有亲水性和亲脂性成分，而注射剂和滴丸剂主要含有亲水性成分。应用主成分分析（PCA）对生药及其制剂的指纹图谱数据进行统计分析。所建立的CFP展示了现有成分的代表性化学特征，可用于丹参及其他含丹参的制剂的鉴别和质量评估。
